LATS2 Antibody

Images

 
Western Blot: LATS2 Antibody [NB200-199] - Detection of Human and Mouse LATS1/ LATS2 by Western Blot. Samples: Whole cell lysate (5, 15, 50 ug) from 293T and (50 ug) from mouse NIH3T3 cells prepared using NETN lysis ...read more
Western Blot: LATS2 Antibody [NB200-199] - Detection of Human LATS2 on HeLa whole cell lysate (5, 15, and 50 microgram) using NB200-199. LATS2 was also immunoprecipitated by another LATS2 antibody.
Proximity Ligation Assay: LATS2 Antibody [NB200-199] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human LATS2 (NB200-199). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

LATS2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Large Tumor Suppressor 2 using the numbering given in entry NP_055387.1 (GeneID 26524).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LATS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Proximity Ligation Assay 1:1000 - 1:20000
Positive Control
HeLa Lysate (NB800-PC1)
LATS2 Lysate (NBL1-12449)
Publications
Read Publications using
NB200-199 in the following applications:

  • IP
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LATS2 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ13161
  • Kinase phosphorylated during mitosis protein
  • KPM
  • Large tumor suppressor homolog 2
  • LATS (large tumor suppressor, Drosophila) homolog 2
  • LATS, large tumor suppressor, homolog 2 (Drosophila)
  • serine/threonine kinase KPM
  • Serine/threonine-protein kinase kpm
  • serine/threonine-protein kinase LATS2
  • Warts-like kinase

Background

This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-199
Species: Hu, Mu
Applications: WB, IP, PLA

Publications for LATS2 Antibody (NB200-199)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(4)
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Filter By Species
Human
(4)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB200-199 Applications Species
Wang J, Sinnett-Smith J, Stevens JV et al. Biphasic regulation of Yes-associated Protein (YAP) cellular localization, phosphorylation and activity by G protein-coupled receptor agonists in intestinal epithelial cells: a novel role for protein kinase D (PKD) J. Biol. Chem. 2016 Jul 01 [PMID: 27369082] (WB, Human) WB Human
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (IP, WB, Human) IP, WB Human
Pefani DE, Latusek R, Pires I et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat. Cell Biol. 2014 Sep 14 [PMID: 25218637]
Reuven N, Adler J, Meltser V, Shaul Y. The Hippo pathway kinase Lats2 prevents DNA damage-induced apoptosis through inhibition of the tyrosine kinase c-Abl. Cell Death Differ 2013 Jul 12 [PMID: 23852372] (WB, Human) WB Human
Zhang L, Iyer J, Chowdhury A et al. KIBRA regulates aurora kinase activity and is required for precise chromosome alignment during mitosis. J Biol Chem 2012 Oct [PMID: 22904328]
Vigneron AM, Vousden KH. An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular senescence. The EMBO Journal. 2011 Nov 8. [PMID: 22068052]
Zhang H, Deo M, Thompson RC et al. Negative regulation of Yap during neuronal differentiation. Dev Biol 2012 Jan [PMID: 22037235]
Zhang K, Rodriguez-Aznar E, Yabuta N et al. Lats2 kinase potentiates Snail1 activity by promoting nuclear retention upon phosphorylation. EMBO J 2011 Sep [PMID: 21952048]
Paramasivam M, Sarkeshik A, Yates JR 3rd et al. Angiomotin family proteins are novel activators of the LATS2 kinase tumor suppressor. Mol Biol Cell 2011 Oct [PMID: 21832154]
Go YM, Kang SM, Roede JR et al. Increased inflammatory signaling and lethality of influenza H1N1 by nuclear thioredoxin-1. PLoS One 2011 [PMID: 21526215]
Show All 14 Publications.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LATS2
Entrez
Uniprot