LATS1 Antibody (3A7) - Azide and BSA Free

Western Blot: LATS1 Antibody (3A7) [H00009113-M09] - S1 monoclonal antibody (M09), clone 3A7. Western Blot analysis of LATS1 expression in HeLa ( Cat # L013V1 ).

Sandwich ELISA: LATS1 Antibody (3A7) [H00009113-M09] - Detection limit for recombinant GST tagged LATS1 is approximately 10ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LATS1 Antibody (3A7) - Azide and BSA Free Summary

• Immunogen: LATS1 (NP_004681, 521 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.PQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKN
• Specificity: LATS1 - LATS, large tumor suppressor, homolog 1 (Drosophila) (3A7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LATS1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LATS1 Antibody (3A7) - Azide and BSA Free

• EC 2.7.11
• h-warts
• Large tumor suppressor homolog 1
• LATS (large tumor suppressor, Drosophila) homolog 1
• LATS, large tumor suppressor, homolog 1 (Drosophila)
• serine/threonine-protein kinase LATS1
• WARTS protein kinase
• WARTSEC 2.7.11.1
• wts

Background

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LATS1
• Entrez:
  • 9113(Human)
• Uniprot:
  • NP_004681(Human)