LAT Antibody (661002) [Alexa Fluor® 594]

Product Discontinued

LAT Antibody (661002) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human LAT
  His30-Gln113
  Accession # O43561
• Specificity: Detects human LAT in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAT

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAT Antibody (661002) [Alexa Fluor® 594]

• 36 kDa phospho-tyrosine adapter protein
• LAT
• LAT1,36 kDa phospho-tyrosine adaptor protein
• linker for activation of T cells
• linker for activation of T-cells family member 1
• p36-38
• pp36

Background

LAT (Linker for activation of T cells; also pp36) is a 36-38 kDa type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TCR, GPVI, and Fc epsilon RI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262 amino acids (aa) in length. It contains a four aa extracellular segment with a 235 aa cytoplasmic domain (aa 28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36 aa upstream of the standard site that may be accompanied by a deletion of aa 114-142. There is also a deletion of aa 88-97, and three substitutions: two aa for aa 215-262, 38 aa for aa 55-262, and 50 aa for aa 35-262. Over aa 30-113, human LAT shares 73% aa identity with mouse LAT.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAT
• Uniprot:
  • O43561(Human)