LASS3 Antibody

Western Blot: LASS3 Antibody [NBP1-02313] - (0.2ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LASS3 Antibody Summary

• Immunogen: Peptide with sequence C-RAERHLIPNGQHGH corresponding to C-Terminus according to NP_849164.2.
• Epitope: C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CERS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Approx. 45 kDa band observed in rat testis lysates (calculated MW of 46.3 kDa band according to human NP_849164.2 and of 47.2 kDa band according to rat NP_001121033.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LASS3 Antibody

• CerS3
• LAG1 homolog, ceramide synthase 3
• LAG1 longevity assurance homolog 3 (S. cerevisiae)
• LAG1 longevity assurance homolog 3
• MGC27091

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LASS3 Antibody (NBP1-02313)(1)

Publication using NBP1-02313

• Mizutani Y, Kihara A, Igarashi Y. LASS3 (longevity assurance homologue 3) is a mainly testis-specific (dihydro)ceramide synthase with relatively broad substrate specificity. Biochem J 2006 Sep 15 [PMID: 16753040]

Bioinformatics

• Gene Symbol: CERS3
• Entrez:
  • 204219(Human)
  • 545975(Mouse)
  • 499174(Rat)
• Uniprot:
  • NP_849164.2(Human)