LAIR1 Antibody (9.1C3)

Product Discontinued

LAIR1 Antibody (9.1C3) Summary

• Immunogen: Human Lymphocytes and Large Granular Lymphocytes (LGL)
• Localization: Cell Surface
• Specificity: Recognizes a single protein of 40kDa, identified as the CD305 (LAIR1). It is an inhibitory receptor, expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. This MAb is expressed on CD34 positive hematopoietic progenitors. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. LAIR1 has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. This MAb is identical to LAIR-1, based on the fact that it not only precipitated a protein of 40kDa but it also binds specifically to LAIR-1 cDNA transfected COS7 cells as well as recognizes LAIR-1 fusion protein in ELISA. LAIR-1 expresses on hematopoietic progenitor, implicating its role in the regulation of hematopoiesis at early stage.
• Isotype: IgG2 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAIR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 19q13.4
  Inhibits NK & ALK Cell Mediated Cytolysis Use Ab without BSA & Azide; nhibits TNF-alpha secretion of NK cells Use Ab without BSA & Azide; nduces Colony Formation of Human Bone Marrow Cells Use Ab without BSA & Azide
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for LAIR1 Antibody (9.1C3)

• CD305 antigen
• CD305
• CD305leukocyte-associated Ig-like receptor 1
• HLAIR1
• LAIR1
• LAIR-1
• leukocyte-associated immunoglobulin-like receptor 1

Background

LAIR1 is a transmembrane glycoprotein(approx. 32kDa) expressed on the majority of human peripheral blood mononuclear leukocytes including T cells, B cells, NK cells, macrophages and dendritic cells. LAIR1 recruits SHP1 and SHP2 tyrosine phosphatases upon activation, and cross-linking of the LAIR1 molecule on NK cells results in strong inhibition of NK cell mediated cytotoxicity. This clone is reported to inhibit NK cell triggering via CD16 molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAIR1
• Uniprot:
  • Q6GTX8()