Lactoferrin Antibody (1A1)

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Immunohistochemistry-Paraffin: Lactoferrin Antibody (1A1) [NBP1-40269] - Analysis of anti-LTF / Lactoferrin antibody with human breast, ductal with secretion at concentration 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P
Clone
1A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lactoferrin Antibody (1A1) Summary

Immunogen
A purified lactoferrin from human milk.
Specificity
Recognizes human lactoferrin, a 78kD globular protein found in body secretions such as milk and saliva and is a member of the transferrin family proteins. Transferrins control the level of free iron in the body, by binding and transporting iron. Lactoferrin does not appear to have a transporting function, instead it has antimicrobial activity, by keeping the levels of iron in body fluids low and thus preventing microbes from acquiring iron. Removing free iron from body fluids and inflamed areas also serve to protect against the damaging effects of free radicals. Lactoferrin is used as a therapeutic and prophylactic treatment of various illnesses, such as cancers and intestinal illnesses. May be used to detect lactoferrin in ELISA assays.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LTF
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry-Paraffin 10 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lactoferrin Antibody (1A1)

  • EC 3.4.21
  • EC 3.4.21.-
  • GIG12
  • growth-inhibiting protein 12
  • HEL110
  • HLF2
  • Kaliocin-1
  • Lactoferricin
  • Lactoferrin
  • Lactoferroxin
  • Lactotransferrin
  • LF
  • LTF
  • neutrophil lactoferrin
  • Talalactoferrin

Background

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

FAQs for Lactoferrin Antibody (NBP1-40269). (Showing 1 - 1 of 1 FAQ).

  1. Is required any pre-treatment in microwave oven before to apply the antibody? Is needed a peculiar inhibition for endogenous peroxidase or is it enough to perform the usual treatment with hydrogen peroxide or methanol? Thanks in advance
    • Our Lactoferrin antibody with catalogue number NBP1-40269 is covered by our 100% guarantee for IHC with paraffin-embedded human samples. We recommend the use of heat induced antigen retrieval in pH 6.0 citrate buffer. Please read our protocol for IHC-P, which includes details of the hydrogen peroxide / methanol method of quenching endogenous peroxide activity. The recommended starting dilution when using this antibody for IHC-P is 10ug/ml.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol LTF
Entrez
Uniprot