Lactate Dehydrogenase C Overexpression Lysate (Native)

Images

 
Western Blot: Lactate Dehydrogenase C Overexpression Lysate (Adult Normal) [NBL1-12473] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lactate Dehydrogenase C.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Lactate Dehydrogenase C Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209516 and based on accession number NM_002301. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens lactate dehydrogenase C (LDHC), transcript variant 1, mRNA.
Gene
LDHC

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
36.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Lactate Dehydrogenase C

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Lactate Dehydrogenase C Overexpression Lysate (Native)

  • Cancer/testis antigen 32
  • CT32EC 1.1.1.27
  • EC 1.1.1
  • lactate dehydrogenase C
  • lactate dehydrogenase C4
  • LDH testis subunit
  • LDH3MGC111073
  • LDH-C
  • LDHX
  • LDH-X
  • L-lactate dehydrogenase C chain

Background

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-48336
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-53421
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-30475
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA
NBP2-01506
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-45978
Species: Hu
Applications: WB, Flow, IHC
NBP1-87069
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-89134
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-42378
Species: Hu
Applications: WB, IHC
NB110-3638
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NB100-410
Species: Hu, Mu
Applications: WB, IP
NBP1-89131
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-32263
Species: Hu
Applications: WB, IHC, IP
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-38770
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for Lactate Dehydrogenase C Lysate (NBL1-12473) (0)

There are no publications for Lactate Dehydrogenase C Lysate (NBL1-12473).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Lactate Dehydrogenase C Lysate (NBL1-12473) (0)

There are no reviews for Lactate Dehydrogenase C Lysate (NBL1-12473). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lactate Dehydrogenase C Lysate (NBL1-12473) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Lactate Dehydrogenase C Products

Bioinformatics Tool for Lactate Dehydrogenase C Lysate (NBL1-12473)

Discover related pathways, diseases and genes to Lactate Dehydrogenase C Lysate (NBL1-12473). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lactate Dehydrogenase C Lysate (NBL1-12473)

Discover more about diseases related to Lactate Dehydrogenase C Lysate (NBL1-12473).
 

Pathways for Lactate Dehydrogenase C Lysate (NBL1-12473)

View related products by pathway.

PTMs for Lactate Dehydrogenase C Lysate (NBL1-12473)

Learn more about PTMs related to Lactate Dehydrogenase C Lysate (NBL1-12473).

Blogs on Lactate Dehydrogenase C

There are no specific blogs for Lactate Dehydrogenase C, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lactate Dehydrogenase C Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol LDHC