Lactate Dehydrogenase C Antibody

Western Blot: Lactate Dehydrogenase C Antibody [NB100-68159] - Lactate Dehydrogenase C Antibody [NB100-68159] (0.03ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68159] - (1ug/ml) staining of paraffin embedded Mouse Testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.

• Reactivity: Mu, Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Lactate Dehydrogenase C Antibody Summary

• Immunogen: Peptide with sequence C-KLGTDSDKEHWKNIH corresponding to internal region according to NP_002292.1, NP_059144.1.
• Epitope: KLGTDSDKEHWKNIH
• Specificity: Both variants represent identical product (NP_002292.1 and NP_059144.1).
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LDHC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 30-35 kDa band observed in mouse testis lysates (calculated MW of 36.6 kDa band according to human NP_002292.1 and 35.9 kDa band according to mouse NP_038608.1). IHC: Strong signal in seminiferous tubules of mouse testis.
• Control:

  Lactate Dehydrogenase C Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase C Antibody

• Cancer/testis antigen 32
• CT32EC 1.1.1.27
• EC 1.1.1
• lactate dehydrogenase C
• lactate dehydrogenase C4
• LDH testis subunit
• LDH3MGC111073
• LDH-C
• LDHX
• LDH-X
• L-lactate dehydrogenase C chain

Background

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lactate Dehydrogenase C Antibody (NB100-68159)(1)

Publication using NB100-68159

• Mazzotta S, Guerranti R, Gozzetti A et al. Increased serum lactate dehydrogenase isoenzymes in Ph-negative chronicmyeloproliferative diseases: a metabolic adaptation? Hematology 2006-08-01 [PMID: 17178662]

Bioinformatics

• Gene Symbol: LDHC
• Entrez:
  • 3948(Human)
  • 16833(Mouse)
• Uniprot:
  • NP_002292.1(Human)