Kynureninase Overexpression Lysate

Images

 
Western Blot: KYNU Overexpression Lysate (Adult Normal) [NBL1-12416] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KYNU.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Kynureninase Overexpression Lysate Summary

Description
Transient overexpression lysate of kynureninase (L-kynurenine hydrolase) (KYNU), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC201559 and based on accession number NM_001032998. The protein contains a C-MYC/DDK Tag.
Gene
KYNU

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Kynureninase Overexpression Lysate

  • EC 3.7.1.3
  • KYNU
  • kynureninase (L-kynurenine hydrolase)
  • Kynureninase
  • L-kynurenine hydrolase

Background

Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol KYNU