Kynureninase Antibody (771312) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 771312
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

Kynureninase Antibody (771312) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase
  Met1-Ser464
  Accession # Q9CXF0
• Specificity: Detects mouse Kynureninase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Kynureninase is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kynureninase Antibody (771312) [Alexa Fluor® 488]

• EC 3.7.1.3
• KYNU
• kynureninase (L-kynurenine hydrolase)
• Kynureninase
• L-kynurenine hydrolase

Background

Kyureninase (KYNU, a hydrolase that acts on dog [Greek: kyon] urine [Greek: ouron]) is a 50-55 kDa member of the kyureninase family of enzymes.  It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages.  Kyureninase participates in the metabolism of tryptophan.  Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD.  Kyureninase catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid.  Mouse Kyureninase is 464 amino acids (aa) in length.  It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer.  There are at least two potential isoform variants.  One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464.  Full-length mouse Kyureninase shares 92% and 83% aa sequence identity with rat and human Kyureninase, respectively.    

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.