Kv7.1 Antibody

Western Blot: Kv7.1 Antibody [NBP1-30119] - Analysis of Kv7.1 in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-30119 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry: Kv7.1 Antibody [NBP1-30119] - (8ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

Control: Kv7.1 Antibody [NBP1-30119] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC, Ctrl
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kv7.1 Antibody Summary

• Description: Novus Biologicals Goat Kv7.1 Antibody (NBP1-30119) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Kv7.1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EQLTVPRRGPDEGS corresponding to C-Terminus according to NP_861463.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000209.2; NP_861463.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KCNQ1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Control
  • Immunohistochemistry 6.0 - 8.0 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1.0 - 3.0 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human heart lysates (calculated MW of 61.5 kDa band according to NP_861463.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kv7.1 Antibody

• ATFB3
• IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1
• JLNS1
• KCNA8
• KCNA8FLJ26167
• KCNA9
• KCNA9Kv1.9
• KCNQ1
• kidney and cardiac voltage dependend K+ channel
• KQT-like 1
• Kv7.1
• KVLQT1
• KVLQT1ATFB1
• LQT
• LQT1
• potassium voltage-gated channel subfamily KQT member 1
• potassium voltage-gated channel, KQT-like subfamily, member 1
• RWS
• slow delayed rectifier channel subunit
• SQT2
• Voltage-gated potassium channel subunit Kv7.1
• WRS

Background

This gene encodes a protein for a voltage-gated potassium channel required for the repolarization phase of the cardiac action potential. The gene product can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation. The gene is located in a region of chromosome 11 that contains a large number of contiguous genes that are abnormally imprinted in cancer and the Beckwith-Wiedemann syndrome. Two alternative transcripts encoding distinct isoforms have been described (from EntrezGene).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kv7.1 Antibody (NBP1-30119)(1)

Publication using NBP1-30119

• Yasuda K, Miyake K, Horikawa Y et al. Variants in KCNQ1 are associated with susceptibility to type 2 diabetes mellitus. Nat Genet 2008-08-17 [PMID: 18711367]

Bioinformatics

• Gene Symbol: KCNQ1
• Entrez:
  • 3784(Human)
  • 16535(Mouse)
  • 84020(Rat)
• Uniprot:
  • P51787(Human)