KRAS Overexpression Lysate (Native)

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Western Blot: KRAS Overexpression Lysate (Adult Normal) [NBL1-12376] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KRAS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KRAS Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201958 and based on accession number NM_004985. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mRNA.
Gene
KRAS

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for KRAS

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KRAS Overexpression Lysate (Native)

  • cellular c-Ki-ras2 proto-oncogene
  • c-Ki-ras
  • C-K-RAS
  • GTPase KRas
  • KI-RAS
  • Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
  • K-Ras 2
  • K-ras p21 protein
  • KRAS1
  • K-RAS2A
  • K-RAS2B
  • KRAS2NS3
  • K-RAS4A
  • K-RAS4B
  • NS
  • oncogene KRAS2
  • PR310 c-K-ras oncogene
  • RASK2c-Kirsten-ras protein
  • transforming protein p21
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq]. Transcript Variant: This variant (b) is composed of five exons and lacks exon 4a which the longer transcript variant (a) includes. This predominant variant (b) has a cds that terminates in exon 4b and encodes isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for KRAS Lysate (NBL1-12376) (0)

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Blogs on KRAS.

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By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

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Bioinformatics

Gene Symbol KRAS