KRAS Antibody (2F8) - BSA Free

Western Blot: KRAS Antibody (2F8) [NBP2-59413] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate

Immunocytochemistry/Immunofluorescence: KRAS Antibody (2F8) [NBP2-59413] - Analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western Blot: KRAS Antibody (2F8) [NBP2-59413] - Lane 1: KRAS recombinant protein, Lane 2: HRAS recombinant protein, Lane 3: NRAS recombinant protein

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

KRAS Antibody (2F8) - BSA Free Summary

• Description: Novus Biologicals Mouse KRAS Antibody (2F8) - BSA Free (NBP2-59413) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-KRAS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human KRAS (1-186aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRAS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for KRAS Antibody (2F8) - BSA Free

• cellular c-Ki-ras2 proto-oncogene
• c-Ki-ras
• C-K-RAS
• GTPase KRas
• KI-RAS
• Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
• K-Ras 2
• K-ras p21 protein
• KRAS1
• K-RAS2A
• K-RAS2B
• KRAS2NS3
• K-RAS4A
• K-RAS4B
• NS
• oncogene KRAS2
• PR310 c-K-ras oncogene
• RASK2c-Kirsten-ras protein
• transforming protein p21
• v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
• v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KRAS Antibody (NBP2-59413)(1)

Publication using NBP2-59413

• Beliakova-Bethell N, Manousopoulou A, Deshmukh S et al. Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency Virology 2022-06-04 [PMID: 35714458]

Bioinformatics

• Gene Symbol: KRAS