KPNA3 Antibody

Western Blot: KPNA3 Antibody [NBP1-31031] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:H129910% SDS PAGE, antibody diluted at 1:1500.

Immunohistochemistry-Paraffin: KPNA3 Antibody [NBP1-31031] - Paraffin-embedded KYSE170 xenograft, using antibody at 1:500 dilution.

Product Discontinued

KPNA3 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human karyopherin alpha 3. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KPNA3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for KPNA3 Antibody

• hSRP1
• importin alpha 4
• Importin alpha Q2
• importin alpha-3
• importin subunit alpha-3
• importin-alpha-Q2
• IPOA4
• karyopherin alpha 3 (importin alpha 4)
• Karyopherin subunit alpha-3
• qip2
• SRP1
• SRP1gamma
• SRP1-gamma
• SRP4

Background

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: KPNA3