KMT2B Antibody

Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KMT2B Antibody Summary

• Immunogen: Peptide with sequence CNCGAKRCRRFLN corresponding to C-Terminus according to NP_055542.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KMT2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10ug/ml
  • Peptide ELISA detection limit 1:128000
• Application Notes: Western blot: Using our routine western blotting protocol we would not expect to reliably detect proteins larger than 250kDa (calculated MWt. of approx. 294KDa according to NP_055542.1).
  IHC: Positive staining seen in paraffin embedded Human Testis and Human Brain Cortex.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KMT2B Antibody

• EC 2.1.1.43
• histone-lysine N-methyltransferase MLL4
• HRX2
• KIAA0304WW domain-binding protein 7
• Lysine N-methyltransferase 2D
• mixed lineage leukemia gene homolog 2
• MLL2
• myeloid/lymphoid or mixed-lineage leukemia 4
• Myeloid/lymphoid or mixed-lineage leukemia protein 4
• Trithorax homolog 2
• trithorax homologue 2
• TRX2
• WBP7
• WBP-7
• WW domain binding protein 7

Background

This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KMT2B Antibody (NB300-864)(1)

Publication using NB300-864

• Huntsman DG, Chin SF, Muleris M et al. MLL2, the second human homolog of the Drosophila trithorax gene, maps to 19q13.1 and is amplified in solid tumor cell lines. Oncogene 1999-12-23 [PMID: 10637508]

Bioinformatics

• Gene Symbol: KMT2B
• Entrez:
  • 9757(Human)
  • 75410(Mouse)
  • 361543(Rat)
• Uniprot:
  • NP_055542.1(Human)