KMT1A/SUV39H1 Knockout 293T Cell Lysate

Product Discontinued

KMT1A/SUV39H1 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1 and Insertion of the selection cassette in exon 1
• Gene: SUV39H1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

Powered by EDIGENE. Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for KMT1A/SUV39H1 Knockout 293T Cell Lysate

• EC 2.1.1.355
• H3-K9-HMTase 1
• Histone H3-K9 methyltransferase 1
• histone-lysine N-methyltransferase SUV39H1
• histone-lysine N-methyltransferase, H3 lysine-9 specific 1
• KMT1A
• KMT1A/SUV39H1
• KMT1AEC 2.1.1.43
• Lysine N-methyltransferase 1A
• MG44
• Position-effect variegation 3-9 homolog
• Su(var)3-9 homolog 1
• suppressor of variegation 3-9 (Drosophila) homolog 1
• suppressor of variegation 3-9 homolog 1 (Drosophila)
• Suppressor of variegation 3-9 homolog 1
• Suppressor of variegation 3-9 homolog 1, Suvar) 3-9 homolog
• SUV39H
• SUV39H1

Background

This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SUV39H1