KIR2.3 Antibody (S25-35)

Western Blot: KIR2.3 Antibody (S25-35) [NBP2-12902] - analysis of Human Cell lysates showing detection of Kir2.3 Potassium Channel protein using Mouse Anti-Kir2.3 Potassium Channel Monoclonal Antibody, Clone S25-35 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.3 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: KIR2.3 Antibody (S25-35) [NBP2-12902] - human hippocampal sections using this antibody.

Product Discontinued

KIR2.3 Antibody (S25-35) Summary

• Immunogen: Fusion protein amino acids 390-445 of human Kir2.3
• Localization: Cell Membrane , Cytoplasmic Vesicle Membrane
• Specificity: Detects approx 45kDa. No cross-reactivity against Kir2.1 or Kir2.2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNJ4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of Kir2.3 Antibody was sufficient for detection of Kir2.3 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KIR2.3 Antibody (S25-35)

• hippocampal inward rectifier potassium channel
• Hippocampal inward rectifier
• HIR
• HIRhIRK2
• HIRK2
• HRK1
• HRK1MGC142066
• Inward rectifier K(+) channel Kir2.3
• inward rectifier K+ channel Kir2.3
• inward rectifier potassium channel 4
• IRK3
• IRK-3
• IRK3MGC142068
• KCNJ4
• KIR2.3
• Potassium channel, inwardly rectifying subfamily J member 4
• potassium inwardly-rectifying channel, subfamily J, member 4

Background

1 ug/mL was sufficient for detection of Kir2.3 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNJ4