Kinesin 5A Antibody - BSA Free

Western Blot: Kinesin 5A Antibody [NBP1-76817] - K562 cell lysate with Kinesin 5A antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/Immunofluorescence: Kinesin 5A Antibody [NBP1-76817] - K562 cells with Kinesin 5A antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody - BSA Free [NBP1-76817] - Immunofluorescence of Kinesin 5A in K562 cells with Kinesin 5A antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Kinesin 5A Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide from near the center of human KIF5. The immunogen is located within amino acids 330 - 380 of KIF5. Amino Acid Squence: KKKYEKEKEKTKAQKETIAK
• Specificity: KIF5 has at least three known isoforms; this antibody recognizes all three.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF5A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/ml
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 118 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Kinesin 5A Peptide (NBP1-76817PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Kinesin 5A Antibody - BSA Free

• D12S1889
• KIF5A variant protein
• kinesin family member 5A
• kinesin heavy chain isoform 5A
• Kinesin heavy chain neuron-specific 1
• kinesin, heavy chain, neuron-specific
• MY050
• Neuronal kinesin heavy chain
• NKHC1
• NKHCspastic paraplegia 10 (autosomal dominant)
• SPG10

Background

The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF5A
• Entrez:
  • 3798(Human)
• Uniprot:
  • Q12840(Human)