KIF1C Antibody

Western Blot: KIF1C Antibody [NB100-57510] - KIF1C is a novel HOOK3-interacting protein.sfGFP-3xFLAG and full length (FL) HOOK3, HOOK3 (1-552), and HOOK3 (553-718) all tagged with sfGFP and 3xFLAG were immunoprecipitated with alpha-FLAG antibodies from transiently transfected HEK-293T cells. Western blots with alpha-HC, alpha-FAM160A2, alpha-KIF1C, and alpha-FLAG antibodies were used to determine which proteins co-immunoprecipitated with each HOOK3 construct.DOI://dx.doi.org/10.7554/eLife.28257.015 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/28257), licensed under a CC-BY license.

Immunoprecipitation: KIF1C Antibody [NB100-57510] - Detection of Human and Mouse KIF1C/LTXS1 on HeLa whole cell lysate using NB100-57510. KIF1C/LTXS1 was also IPed by rabbit anti-KIF1C/LTXS1 antibodies NB100-57507, NB100-57508 and NB100-57509.

KIF1C is a novel HOOK3-interacting protein.sfGFP-3×FLAG and full length (FL) HOOK3, HOOK3 (1–552), and HOOK3 (553–718) all tagged with sfGFP and 3×FLAG were immunoprecipitated with alpha -FLAG antibodies from transiently transfected HEK-293T cells. Western blots with alpha -HC, alpha -FAM160A2, alpha -KIF1C, and alpha -FLAG antibodies were used to determine which proteins co-immunoprecipitated with each HOOK3 construct.DOI://dx.doi.org/10.7554/eLife.28257.015

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

KIF1C Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1053 and the C-terminus (residue 1103) of human kinesin family member 1C (lethal toxin sensitivity 1) using the numbering given in entry NP_006603.2 (GeneID 10749).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF1C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KIF1C Antibody

• KIAA0706
• kinesin family member 1C
• kinesin-like protein KIF1C
• LTXS1

Background

The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF1C, also known as LTXS1 (lethal toxin sensitivity), has been found to be localized to the golgi and the endoplasmic reticulum (ER) and play a role in vesicle transport between the golgi and ER. Alternate identifiers of KIF1C include kinesin family member 1C, kinesin-like protein KIF1C and KIAA0706.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIF1C Antibody (NB100-57510)(1)

Publication using NB100-57510

• Redwine WB, DeSantis ME, Hollyer I et al. The human cytoplasmic dynein interactome reveals novel activators of motility Elife 2017-07-18 [PMID: 28718761] (WB, Human)

Review for KIF1C Antibody (NB100-57510) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 12/08/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: isolated adult cardiomyocytes
• Species: Mouse
• Lot: A1

Comments

• Comments: Tried both PFA and methanol fixation. Incubated primary at dilutions of 1:50 and 1:100, and incubated at room temp for 3 hours and at 4C overnight- all different conditions gave no signal using a alexa fluor 488 secondary.

Bioinformatics

• Gene Symbol: KIF1C
• Entrez:
  • 10749(Human)
  • 16562(Mouse)
• Uniprot:
  • NP_006603.2(Human)