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Kelch-Like 3 Antibody

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Western Blot: Kelch-Like 3 Antibody [NBP3-03236] - Analysis of extracts of various cell lines, using Kelch-Like 3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western Blot: Kelch-Like 3 Antibody [NBP3-03236] - Analysis of extracts of various cell lines, using Kelch-Like 3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Kelch-Like 3 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human Kelch-Like 3 (NP_059111.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLHL3
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for Kelch-Like 3 Antibody

  • FLJ40871
  • kelch-like 3 (Drosophila)
  • kelch-like protein 3
  • KIAA1129kelch (Drosophila)-like 3
  • MGC44594

Background

KLHL3, also known as Kelch-like protein 3; has 3 isoforms, a 65 kDa 587 amino acid isoform A, a 61 kDa 555 amino acid isoform B, and a 56 kDa 505 amino acid isoform C; acts as a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that plays a role of regulator in ion transport of the distal nephron. The BCR(KLHL3) complex may regulate the SLC12A3/NCC subcellular location at the cell membrane by mediating ubiquitination of SLC12A3/NCC. There has been research revolving around the KLHL3 protein involvement in pharyngitis and pseudohypoaldosteronism. The protein has been shown to interact with OPRD1, GNAO1, SYT2, SYNCRIP, and GNAI1 proteins in protein ubiquitination, renal sodium ion absorption, and distal tubule morphogenesis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).
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Flow Cytometry: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.
Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.
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Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WNK4 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence
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Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.
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Immunohistochemistry: KIF20A Antibody [NBP2-87689] - Immunohistochemistry with HeLa, Vera, HeLa transfected with mouse construct tissue
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Western Blot: HSD11B2 Antibody [NBP2-37898] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue
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Bioinformatics

Gene Symbol KLHL3