Kelch-Like 3 Antibody

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Western Blot: Kelch-Like 3 Antibody [NBP3-03236] - Analysis of extracts of various cell lines, using Kelch-Like 3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Kelch-Like 3 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human Kelch-Like 3 (NP_059111.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLHL3
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for Kelch-Like 3 Antibody

  • FLJ40871
  • kelch-like 3 (Drosophila)
  • kelch-like protein 3
  • KIAA1129kelch (Drosophila)-like 3
  • MGC44594

Background

KLHL3, also known as Kelch-like protein 3; has 3 isoforms, a 65 kDa 587 amino acid isoform A, a 61 kDa 555 amino acid isoform B, and a 56 kDa 505 amino acid isoform C; acts as a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that plays a role of regulator in ion transport of the distal nephron. The BCR(KLHL3) complex may regulate the SLC12A3/NCC subcellular location at the cell membrane by mediating ubiquitination of SLC12A3/NCC. There has been research revolving around the KLHL3 protein involvement in pharyngitis and pseudohypoaldosteronism. The protein has been shown to interact with OPRD1, GNAO1, SYT2, SYNCRIP, and GNAI1 proteins in protein ubiquitination, renal sodium ion absorption, and distal tubule morphogenesis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Kelch-Like 3 Antibody (NBP3-03236)

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Bioinformatics

Gene Symbol KLHL3