Keap1 Antibody (333116) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clone: 333116
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Keap1 Antibody (333116) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Keap1
  Ala90-Ile250
  Accession # Q14145
• Specificity: Detects human, mouse and rat Keap1 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Keap1 Antibody (333116) [Alexa Fluor® 350]

• INrf2
• INrf2MGC9454
• Keap1
• kelch-like ECH-associated protein 1
• Kelch-like protein 19
• KIAA0132MGC4407
• KLHL19Cytosolic inhibitor of Nrf2
• MGC10630
• MGC1114
• MGC20887

Background

Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.