![Western Blot: KCNK4 Antibody [NBP2-76938] - Western blot analysis of K2P4.1 on different lysates using anti-K2P4.1 antibody at 1/500 dilution.Positive control:Lane1: Mouse colon Lane2: Rat kidney](http://images.novusbio.com/fullsize2/KCNK4-Antibody-Western-Blot-NBP2-76938-img0001.jpg "Western Blot: KCNK4 Antibody [NBP2-76938] - Western blot analysis of K2P4.1 on different lysates using anti-K2P4.1 antibody at 1/500 dilution.Positive control:Lane1: Mouse colon Lane2: Rat kidney")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide within C-terminal human KCNK4. (SwissProt: Q9NYG8 Human) |
| Localization | Cell membrane. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KCNK4 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.5%BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Western Blot: KCNK3 Antibody (S374-48) [NBP2-42202] - Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa.](http://images.novusbio.com/images2/KCNK3-Antibody-(S374-48)-Western-Blot-NBP2-42202-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) TASK1 Potassium Channel Antibody. (D) Composite.](http://images.novusbio.com/images2/KCNK3 Antibody (S374-48)-Immunocytochemistry-Immunofluorescence-NBP2-42202-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK3 Antibody (S374-48) [NBP2-42202] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (NBP2-42202). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (NBP2-42202) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TASK1 Potassium Channel Antibody (D) Composite.](http://images.novusbio.com/images2/KCNK3-Antibody-(S374-48)-Immunocytochemistry-Immunofluorescence-NBP2-42202-img0005.jpg)
![Immunohistochemistry: KCNK9 Antibody [NBP2-84109] - Immunohistochemistry with prostate cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-KCNK9 antibody](http://images.novusbio.com/images2/KCNK9-Antibody-Immunohistochemistry-NBP2-84109-img0001.jpg)
![Immunohistochemistry: KCNK9 Antibody [NBP2-84109] - Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-KCNK9 antibody](http://images.novusbio.com/images2/KCNK9-Antibody-Immunohistochemistry-NBP2-84109-img0002.jpg)
![Western Blot: KCNK9 Antibody [NBP2-84109] - Host: Rabbit. Target Name: KCNK9. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/images2/KCNK9-Antibody-Western-Blot-NBP2-84109-img0004.jpg)
![Immunohistochemistry-Paraffin: KCNK5 Antibody [NBP2-32432] - Staining of human kidney shows positivity in a subset of renal tubules.](http://images.novusbio.com/images2/KCNK5-Antibody-Immunohistochemistry-Paraffin-NBP2-32432-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK5 Antibody [NBP2-32432] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.](http://images.novusbio.com/images2/KCNK5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32432-img0008.jpg)
![Western Blot: KCNK1 Antibody [NBP2-76940] - Western blot analysis of KCNK1 on different cell lysate using anti-KCNK1 antibody at 1/500 dilution.Positive control:Lane1: Human placentaLane2: Human liver](http://images.novusbio.com/images2/KCNK1-Antibody-Western-Blot-NBP2-76940-img0001.jpg)
![Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/KCNK1-Antibody-Immunohistochemistry-NBP2-76940-img0002.jpg)
![Immunohistochemistry: KCNK1 Antibody [NBP2-76940] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KCNK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/KCNK1-Antibody-Immunohistochemistry-NBP2-76940-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK18 Antibody [NBP1-77097] - Immunofluorescence of TRESK in Rat Brain cells with TRESK antibody at 20 ug/mL.](http://images.novusbio.com/images2/KCNK18-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77097-img0003.jpg)
![Western Blot: KCNK18 Antibody [NBP1-77097] - Rat brain tissue lysate with KCNK18 antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/images2/KCNK18-Antibody-Western-Blot-NBP1-77097-img0001.jpg)
![Immunohistochemistry-Paraffin: KCNK18 Antibody [NBP1-77097] - Rat brain tissue with KCNK18 antibody at 5 ug/mL.](http://images.novusbio.com/images2/KCNK18-Antibody-Immunohistochemistry-Paraffin-NBP1-77097-img0002.jpg)
![Chromatin Immunoprecipitation: TEF1 Antibody [NBP2-94035] - Analysis of extracts of HeLa cell line, using TEF1 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/images2/TEF1-Antibody-Chromatin-Immunoprecipitation-NBP2-94035-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-94035] - Analysis of MCF7 cells using TEF1 .](http://images.novusbio.com/images2/TEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94035-img0002.jpg)
![Western Blot: TEF1 Antibody [NBP2-94035] - Analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/images2/TEF1-Antibody-Western-Blot-NBP2-94035-img0011.jpg)
![Western Blot: KvBeta2 Antibody [NBP1-80271] - Antibody Titration: 1 ug/ml Human HepG2.](http://images.novusbio.com/images2/KvBeta2-Antibody-Western-Blot-NBP1-80271-img0007.jpg)
![Western Blot: KvBeta2 Antibody [NBP1-80271] - Reccomended Titration: 1.25 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/images2/KvBeta2-Antibody-Western-Blot-NBP1-80271-img0008.jpg)
![Immunohistochemistry-Paraffin: KvBeta2 Antibody [NBP1-80271] - KvBeta2 Antibody Sample Type: P48 Mouse. Dilution: 1:2000.](http://images.novusbio.com/images2/KvBeta2-Antibody-Immunohistochemistry-Paraffin-NBP1-80271-img0004.jpg)
![Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP1-31133] - Paraffin-embedded hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/TBP-like-protein-TLP-Antibody-Immunohistochemistry-Paraffin-NBP1-31133-img0002.jpg)
![Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/images2/TBP-like-protein-TLP-Antibody-Western-Blot-NBP1-31133-img0003.jpg)
![Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/images2/TBP-like-protein-TLP-Antibody-Western-Blot-NBP1-31133-img0004.jpg)
![Western Blot: KCNK12 Antibody [NBP2-81727] - Analysis of KCNK12 in rat brain tissue lysate with KCNK12 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/images2/KCNK12-Antibody-Western-Blot-NBP2-81727-img0001.jpg)
![Immunohistochemistry: KCNK12 Antibody [NBP2-81727] - Immunohistochemistry of KCNK12 in mouse brain tissue with KCNK12 antibody at 5 ug/mL.](http://images.novusbio.com/images2/KCNK12-Antibody-Immunohistochemistry-NBP2-81727-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: KCNK12 Antibody [NBP2-81727] - Immunofluorescence of KCNK12 in mouse brain tissue with KCNK12 antibody at 20 ug/mL.](http://images.novusbio.com/images2/KCNK12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81727-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-1617] - TRPV1 staining in rat trigeminal ganglion. Image courtesy of Dr. Norman Capra, University of Maryland](http://images.novusbio.com/images2/Vanilloid-R1-TRPV1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1617-img0003.jpg)
![Western Blot: Vanilloid R1/TRPV1 Antibody [NB100-1617] - Nociceptive behavior and CBS, TRPA1, TRPC6 and TRPV1 protein expression of the dorsal root ganglia obtained from normoglycemic and diabetic rats at 21 days post-streptozotocin injection. TRPV1 protein expression in normoglycemic and diabetic rats 21 days after streptozotocin injection. Data were normalized against Beta-actin and are expressed as the mean?+-?SEM of 5-6 animals. *Significantly different from control or normoglycemic (NG) group, #significantly different from vehicle (Veh, diabetic rats) group and &significantly different from SKF-96365 (SKF) group (P?<?0.05) determined by one-way ANOVA followed by the Student-Newman-Keuls' test Image collected and cropped by CiteAb from the following publication (https://bmcneurosci.biomedcentral.com/articles/10.1186/s12868-018-0483-3) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Vanilloid-R1-TRPV1-Antibody-Western-Blot-NB100-1617-img0007.jpg)
![Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NB100-1617] - TRPV1 Antibody [NB100-1617] - Dorsal Root Ganglion](http://images.novusbio.com/images2/Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-NB100-1617-img0001.jpg)
Secondary Antibodies |
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Diseases for KCNK4 Antibody (NBP2-76938)Discover more about diseases related to KCNK4 Antibody (NBP2-76938).
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PTMs for KCNK4 Antibody (NBP2-76938)Learn more about PTMs related to KCNK4 Antibody (NBP2-76938).
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| Gene Symbol | KCNK4 |
![Immunohistochemistry: KCNK4 Antibody [NBP2-76938] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/KCNK4-Antibody-Immunohistochemistry-NBP2-76938-img0003.jpg "Immunohistochemistry: KCNK4 Antibody [NBP2-76938] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: KCNK4 Antibody [NBP2-76938] - Flow cytometric analysis of HepG2 cells with K2P4.1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/KCNK4-Antibody-Flow-Cytometry-NBP2-76938-img0004.jpg "Flow Cytometry: KCNK4 Antibody [NBP2-76938] - Flow cytometric analysis of HepG2 cells with K2P4.1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry: KCNK4 Antibody [NBP2-76938] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/KCNK4-Antibody-Immunohistochemistry-NBP2-76938-img0002.jpg "Immunohistochemistry: KCNK4 Antibody [NBP2-76938] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.")
![Western Blot: TREK 1 Antibody [NB110-41535] - Detection of TREK 1 in membrane lysate fraction of human brain tissue.](http://images.novusbio.com/images2/TREK-1-Antibody-Western-Blot-NB110-41535-img0003.jpg)
![ELISA: KCNK10 Antibody (1C1) [H00054207-M03] - Detection limit for recombinant GST tagged KCNK10 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/images2/KCNK10-Antibody-(1C1)-ELISA-H00054207-M03-img0001.jpg)
![Immunohistochemistry-Paraffin: Potassium channel subfamily K member 6 Antibody [NBP2-34063] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/images2/Potassium-channel-subfamily-K-member-6-Antibody-Immunohistochemistry-Paraffin-NBP2-34063-img0001.jpg)
![Immunohistochemistry: KCNK13 Antibody [NBP2-41132] - Human brain tissue with KCNK13 antibody at 20 ug/mL.](http://images.novusbio.com/images2/KCNK13-Antibody-Immunofluorescence-NBP2-41132-img0003.jpg)
![Immunohistochemistry-Paraffin: KCNK13 Antibody [NBP2-41132] - Human brain tissue with KCNK13 antibody at 5 ug/mL.](http://images.novusbio.com/images2/KCNK13-Antibody-Immunohistochemistry-Paraffin-NBP2-41132-img0002.jpg)
![Western Blot: KCNK13 Antibody [NBP2-41132] - Analysis of KCNK13 in rat brain tissue lysate with KCNK13 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/images2/KCNK13-Antibody-Western-Blot-NBP2-41132-img0001.jpg)
![Western Blot: TYRP1 Antibody [NBP1-88370] - Analysis in control (vector only transfected HEK293T lysate) and TYRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/TYRP1-Antibody-Western-Blot-NBP1-88370-img0011.jpg)
![Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Analysis in human skin and cerebral cortex tissues. Corresponding TYRP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/TYRP1-Antibody-Immunohistochemistry-Paraffin-NBP1-88370-img0012.jpg)
![Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/images2/TYRP1-Antibody-Immunohistochemistry-Paraffin-NBP1-88370-img0013.jpg)
![Western Blot: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in PC12 cell lysate.](http://images.novusbio.com/images/TRPM8-Antibody-Western-Blot-NBP1-97311-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP1-97311] - TRPM8 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/TRPM8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97311-img0004.jpg)
![Immunohistochemistry: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/images2/TRPM8-Antibody-Immunohistochemistry-NBP1-97311-img0005.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/images2/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg)
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")