KCMF1 Antibody

Western Blot: KCMF1 Antibody [NBP1-84284] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human colon, kidney, liver and testis using Anti-KCMF1 antibody NBP1-84284 (A) shows similar protein distribution across tissues to independent antibody NBP1-84283 (B).

Western Blot: KCMF1 Antibody [NBP1-84284] - Analysis in human cell line BEWO.

Immunocytochemistry/Immunofluorescence: KCMF1 Antibody [NBP1-84284] - staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human liver shows no cytoplasmic postivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human testis shows weak to moderate cytoplasmic postivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: KCMF1 Antibody [NBP1-84284] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Product Discontinued

KCMF1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LSGVRRSAGGQLNSSGPSASQLQQLQMQLQLERQHAQAARQQLETARNATRRTNTSSVTTTITQSTATTNIANTESSQQTLQNSQFLLTRL
• Predicted Species: Mouse (91%), Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCMF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
  • Western Blot 1:100-1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for KCMF1 Antibody

• DEBT91
• DKFZp434L1021
• EC 6.3.2
• EC 6.3.2.-
• FGF-induced in gastric cancer
• FGF-induced ubiquitin-protein ligase in gastric cancers
• FIGC
• PCMFdifferentially expressed in branching tubulogenesis 91
• potassium channel modulatory factor 1
• Potassium channel modulatory factor
• zinc finger, ZZ domain containing 1
• ZZ-type zinc finger-containing protein 1
• ZZZ1E3 ubiquitin-protein ligase KCMF1

Background

KCMF1 has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCMF1