KCC2/SLC12A5 Overexpression Lysate (Native)

Images

 
Western Blot: SLC12A5 Overexpression Lysate (Adult Normal) [NBL1-16006] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KCC2/SLC12A5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223680 and based on accession number NM_020708. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens solute carrier family 12 (potassium-chloride transporter), member 5 (SLC12A5), transcript variant 2, mRNA.
Gene
SLC12A5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
123.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KCC2/SLC12A5 Overexpression Lysate (Native)

  • Electroneutral potassium-chloride cotransporter 2
  • hKCC2
  • KCC2
  • KCC2K-Cl cotransporter 2
  • KIAA1176erythroid K-Cl cotransporter 2
  • Neuronal K-Cl cotransporter
  • SLC12A5
  • solute carrier family 12 (potassium/chloride transporter), member 5
  • solute carrier family 12 member 5

Background

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2, also known as KCC2b) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for KCC2/SLC12A5 Lysate (NBL1-16006) (0)

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Bioinformatics

Gene Symbol SLC12A5
Entrez