Karyopherin (importin) beta 3 Antibody

Product Discontinued

Karyopherin (importin) beta 3 Antibody Summary

• Immunogen: Synthetic peptide PEDQVGKLEATEN-C, representing the N-Terminus of the human protein according to NP_002262.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IPO5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA detection limit 1:4000
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave no signal but low background in Human Testis lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Karyopherin (importin) beta 3 Antibody

• DKFZp686O1576
• FLJ43041
• IMB3
• imp5
• importin 5
• importin beta-3 subunit
• Importin subunit beta-3
• importin-5
• karyopherin (importin) beta 3
• Karyopherin beta-3
• KPNB3
• MGC2068
• RAN binding protein 5
• Ran_GTP binding protein 5
• Ran-binding protein 5
• RANBP5

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IPO5
• Entrez:
  • 3843(Human)
• Uniprot:
  • NP_002262.(Human)