JunB/AP-1 Antibody (SD081-08)

Western Blot: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Western blot analysis of JunB/AP-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti

Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Staining JunB in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-JunB/AP-1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of JunB on different lysates using anti-JunB antibody at 1/1,000 dilution. Positive control: Lane 1: U937 Lane 2: THP-1

Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Staining JunB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-JunB/AP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-JunB/AP-1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-JunB/AP-1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

JunB/AP-1 Antibody (SD081-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal human JunB/AP-1. (SwissProt: P17275 Human; SwissProt: P09450 Mouse; SwissProt: P24898 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: JUNB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for JunB/AP-1 Antibody (SD081-08)

• activator protein 1
• AP-1
• jun B proto-oncogene
• JunB
• transcription factor jun-B

Background

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JUNB