JunB/AP-1 Antibody (SD081-08)

Images

 
Western Blot: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Western blot analysis of JunB/AP-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Staining JunB in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-JunB/AP-1 antibody washed with ddH2O and PBS, and then probed with ...read more
Western Blot: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of JunB on different lysates using anti-JunB antibody at 1/1,000 dilution. Positive control: Lane 1: U937 Lane 2: THP-1
Immunocytochemistry/ Immunofluorescence: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Staining JunB in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-JunB/AP-1 antibody. The section was pre-treated using heat ...read more
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-JunB/AP-1 antibody washed with ddH2O and PBS, and then probed with the primary ...read more
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (SD081-08) [NBP2-67788] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-JunB/AP-1 antibody washed with ddH2O and PBS, and then probed with the primary ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-67788
    • Availability
      Product Discontinued

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JunB/AP-1 Antibody (SD081-08) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within N-terminal human JunB/AP-1. (SwissProt: P17275 Human; SwissProt: P09450 Mouse; SwissProt: P24898 Rat)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
JUNB
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for JunB/AP-1 Antibody (SD081-08)

  • activator protein 1
  • AP-1
  • jun B proto-oncogene
  • JunB
  • transcription factor jun-B

Background

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol JUNB