JNK3 Antibody (10E4A4)

Images

 
Western Blot: JNK3 Antibody (10E4A4) [NBP1-51544] - Western blot analysis using MAPK10 mouse mAb against NIH/3T3 (1) and SKN-SH (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: JNK3 Antibody (10E4A4) [NBP1-51544] - Immunofluorescence staining of methanol-fixed A431 (left) and Hela (right) cells showing cytoplasmic and membrane localization.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
10E4A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

JNK3 Antibody (10E4A4) Summary

Immunogen
Purified recombinant fragment of human MAPK10 (aa28-233) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAPK10
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for JNK3 Antibody (10E4A4)

  • c-Jun N-terminal kinase 3
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK3 alpha protein kinase
  • JNK3
  • JNK3A
  • JNK3FLJ12099
  • MAP kinase 10
  • MAP kinase p49 3F12
  • MAPK 10
  • MAPK10
  • MGC50974
  • mitogen-activated protein kinase 10
  • p493F12
  • p54bSAPK
  • PRKM10FLJ33785
  • SAPK1 beta
  • stress activated protein kinase beta
  • Stress-activated protein kinase JNK3

Background

C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in heart and nervous system nervous. JNK3 has been implicated in stress-induced neuronal apoptosis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
MAB1846
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-47839
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF4559
Species: Hu
Applications: WB
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB500-138
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37542
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-37568
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-68874
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
NBP1-88419
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-00764
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP1-81575
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56077
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NBP1-91946
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for JNK3 Antibody (NBP1-51544) (0)

There are no publications for JNK3 Antibody (NBP1-51544).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for JNK3 Antibody (NBP1-51544) (0)

There are no reviews for JNK3 Antibody (NBP1-51544). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for JNK3 Antibody (NBP1-51544) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional JNK3 Products

Bioinformatics Tool for JNK3 Antibody (NBP1-51544)

Discover related pathways, diseases and genes to JNK3 Antibody (NBP1-51544). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for JNK3 Antibody (NBP1-51544)

Discover more about diseases related to JNK3 Antibody (NBP1-51544).
 

Pathways for JNK3 Antibody (NBP1-51544)

View related products by pathway.

PTMs for JNK3 Antibody (NBP1-51544)

Learn more about PTMs related to JNK3 Antibody (NBP1-51544).
 

Research Areas for JNK3 Antibody (NBP1-51544)

Find related products by research area.

Blogs on JNK3

There are no specific blogs for JNK3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our JNK3 Antibody (10E4A4) and receive a gift card or discount.

Bioinformatics

Gene Symbol MAPK10
Entrez