JNK3 Antibody (10E4A4)

Western Blot: JNK3 Antibody (10E4A4) [NBP1-51544] - Analysis using MAPK10 mouse mAb against NIH/3T3 (1) and SKN-SH (2) cell lysate.

Immunocytochemistry/Immunofluorescence: JNK3 Antibody (10E4A4) [NBP1-51544] - Immunofluorescence staining of methanol-fixed A431 (left) and Hela (right) cells showing cytoplasmic and membrane localization.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 10E4A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

JNK3 Antibody (10E4A4) Summary

• Description: Novus Biologicals Mouse JNK3 Antibody (10E4A4) (NBP1-51544) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human JNK3 (aa28-233) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK10
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for JNK3 Antibody (10E4A4)

• c-Jun N-terminal kinase 3
• EC 2.7.11
• EC 2.7.11.24
• JNK3 alpha protein kinase
• JNK3
• JNK3A
• JNK3FLJ12099
• MAP kinase 10
• MAP kinase p49 3F12
• MAPK 10
• MAPK10
• MGC50974
• mitogen-activated protein kinase 10
• p493F12
• p54bSAPK
• PRKM10FLJ33785
• SAPK1 beta
• stress activated protein kinase beta
• Stress-activated protein kinase JNK3

Background

C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in heart and nervous system nervous. JNK3 has been implicated in stress-induced neuronal apoptosis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK10