JNK1 [p Thr183, p Thr221] Antibody (ST500)

Western Blot: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of JNK1/2/3 [p Thr183, p Thr183, p Thr221] on different lysates with Rabbit anti- JNK1/2/3 [p Thr183, p Thr183, p Thr221] antibody (untreated Lane 2: Hela cell lysate, treated with Anisomycin Lane 3: A431 cell lysate, untreated Lane 4: A431 cell lysate, treated with UV40 Lane 5: 293 cell lysate, untreated Lane 6: 293 cell lysate, treated with UV40 Lane 7: Hela cell lysate, untreated Lane 8: Hela cell lysate, treated with UV40 Lysates/proteins at 10 ug/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Phospho-JNK1 [p Thr183, p Thr183, p Thr221] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of Hela cells with Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the second

Western Blot: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of Phospho-JNK1/2/3(T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 cell lysate, treated with Anisomycin Lane 2: NIH/3T3 cell lysate, untreated

Immunocytochemistry/Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human endometrium tissue with Rabbit anti-JNK1 [p Thr183, p Thr183, p Thr221) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

JNK1 [p Thr183, p Thr221] Antibody (ST500) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr183 + Thr183 + Thr221 of Human JNK1/2/3 aa 161-204 / 427. (SwissProt: P45983 Human; SwissProt: P45984 Human; SwissProt: P53779 Human; SwissProt: Q61831 Mouse; SwissProt: Q91Y86 Mouse; SwissProt: Q9WTU6 Mouse; SwissProt: P49185 Rat; SwissProt: P49186 Rat; SwissProt: P49187 Rat)
• Modification: p Thr183, p Thr221
• Localization: Cytoplasm, Membrane, Mitochondrion, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for JNK1 [p Thr183, p Thr221] Antibody (ST500)

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• MAPK8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8
• PRKM8JNK
• protein kinase JNK1
• SAPK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

JNK1, also known as MAPK1, is a JNK protein subgroup familymenber of the mitogen-activated protein kinase (MAPK) superfamily and is part of a signal transduction cascade that is activated by cytokines and by exposure to environmental stresses. Components of the JNK (c-Jun N-terminal kinase) signaling pathway have been shown to interact with the insulin signaling pathway and play a pivotal role in lifespan regulation and stress resistance in many diverse organisms. Therefore, JNK1 plays a role in regulating both life span and stress resistance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for JNK1 Antibody (NBP2-67514) (1)

Average Rating: 2

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot WB Human 10/17/2021

Summary

• Application: Western Blot
• Sample Tested: U251 glioma cell line; whole cell lysate
• Species: Human
• Lot: HN1207

Comments

• Comments: ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Bioinformatics

• Gene Symbol: MAPK8