JNK1 [p Thr183, p Thr221] Antibody (ST500)

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Western Blot: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of Phospho-JNK1/2/3(T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/1,000 dilution. ...read more
Immunocytochemistry/ Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were ...read more
Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with ...read more
Flow Cytometry: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of Hela cells with Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without ...read more
Immunocytochemistry/ Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were ...read more
Immunocytochemistry/ Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were ...read more
Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with ...read more
Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3(T183+T183+T221) antibody. Counter stained with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
ST500
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

JNK1 [p Thr183, p Thr221] Antibody (ST500) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr183 + thr183 + thr221 of human jnk1/2/3 (SwissProt: P45983 Human;SwissProt: P45984 Human;SwissProt: P53779 Human;SwissProt: Q61831 Mouse;SwissProt: Q91Y86 Mouse;SwissProt: Q9WTU6 Mouse;SwissProt: P49185 Rat;SwissProt: P49186 Rat;SwissProt: P49187 Rat)
Modification
p Thr183, p Thr221
Localization
Cytoplasm, Nucleus, Membrane, Mitochondrion.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MAPK8
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation
  • Western Blot 1:1000 - 1:2000
Reviewed Applications
Read 1 Review rated 2
using
NBP2-67514 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH 7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for JNK1 [p Thr183, p Thr221] Antibody (ST500)

  • c-Jun N-terminal kinase 1
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JNK1
  • JNK1JNK1A2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • MAPK 8
  • MAPK8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 beta2
  • mitogen-activated protein kinase 8
  • PRKM8
  • PRKM8JNK
  • protein kinase JNK1
  • SAPK1
  • SAPK1JNK21B1/2
  • Stress-activated protein kinase 1
  • Stress-activated protein kinase JNK1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JNK1 Antibody (NBP2-67514) (0)

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Review for JNK1 Antibody (NBP2-67514) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-67514:
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Images Ratings Applications Species Date Details
Western Blot JNK1 NBP2-67514
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2
reviewed by:
Pauline Jeannot
WB Human 10/17/2021
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Summary

ApplicationWestern Blot
Sample TestedU251 glioma cell line; whole cell lysate
SpeciesHuman
LotHN1207

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

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FAQs for JNK1 Antibody (NBP2-67514) (0)

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Recent Reviews

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Pauline Jeannot
10/17/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAPK8