JNK1/2 [p Tyr185, p Thr183] Antibody

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Western Blot: JNK1/2 [p Thr183, p Tyr185] Antibody [NSB682] - Western Blot analysis using rabbit anti-JNK [pTpY183/185] Polyclonal Antibody. Western blot analysis. JNK [pTpY183/185] phosphospecific antibody. 293 and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

JNK1/2 [p Tyr185, p Thr183] Antibody Summary

Immunogen
Synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence. This region is conserved among many species including human, rat, mouse, chick, nematode (Caenorhabditis elegans), and fly (Drosophila melanogaster).
Epitope
JNK1&2 phosphorylated at threonine 183 and tyrosine 185.
Modification
p Thr183, p Tyr185
Specificity
Phosphorylation site-specific selective for the dually phosphorylated form of the c-Jun N-terminal Kinase (JNK)/Stress-Activated Protein Kinase (SAPK) enzymes containing a phosphate on threonine 183 and tyrosine 185 (human JNK 1 + 2). The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated JNK1&2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
In WB predicted molecular weight: 49-55 kDa. Can be blocked with JNK 1 + 2 phospho T183 / Y185 and non-phospho peptides (pair)or PC12 whole cell lysate, + / - Sorbitol treatment.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with a wide range of other species due to sequence identity. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for JNK1/2 [p Tyr185, p Thr183] Antibody

  • c-Jun N-terminal kinase 1
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JNK1JNK1A2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • MAPK 8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 beta2
  • mitogen-activated protein kinase 8
  • PRKM8JNK
  • protein kinase JNK1
  • SAPK1JNK21B1/2
  • Stress-activated protein kinase 1
  • Stress-activated protein kinase JNK1

Background

JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAPK8
Entrez