JNK1/2 [p Tyr185, p Thr183] Antibody

Product Discontinued

JNK1/2 [p Tyr185, p Thr183] Antibody Summary

• Immunogen: Synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence. This region is conserved among many species including human, rat, mouse, chick, nematode (Caenorhabditis elegans), and fly (Drosophila melanogaster).
• Epitope: JNK1&2 phosphorylated at threonine 183 and tyrosine 185.
• Modification: p Thr183, p Tyr185
• Specificity: Phosphorylation site-specific selective for the dually phosphorylated form of the c-Jun N-terminal Kinase (JNK)/Stress-Activated Protein Kinase (SAPK) enzymes containing a phosphate on threonine 183 and tyrosine 185 (human JNK 1 + 2). The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated JNK1&2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: In WB predicted molecular weight: 49-55 kDa. Can be blocked with JNK 1 + 2 phospho T183 / Y185 and non-phospho peptides (pair)or PC12 whole cell lysate, + / - Sorbitol treatment.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with a wide range of other species due to sequence identity. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for JNK1/2 [p Tyr185, p Thr183] Antibody

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8JNK
• protein kinase JNK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK8
• Entrez:
  • 5599(Human)