JNK1/2/3 Antibody (252355) [Janelia Fluor® 549]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 252355
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 549

JNK1/2/3 Antibody (252355) [Janelia Fluor® 549] Summary

• Immunogen: E. coli-derived recombinant human JNK
  Ser2-Ile384
  Accession # P45983
• Specificity: Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant JNK1, JNK2, and JNK3.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK8IP3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for JNK1/2/3 Antibody (252355) [Janelia Fluor® 549]

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8JNK
• protein kinase JNK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun Nterminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK8IP3