Jagged 2 Antibody (746513) [Alexa Fluor™ Plus 405]

• Reactivity: Mu
• Clone: 746513
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 405

Jagged 2 Antibody (746513) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects mouse Jagged 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Jagged 1, rhJagged 2, recombinant rat Jagged 1 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 2 Antibody (746513) [Alexa Fluor™ Plus 405]

• HJ2
• JAG2
• Jagged 2
• Jagged2
• protein jagged-2
• SER2

Background

Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins, and thereby regulate cell fate determinations during development (2-6). It is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a 1059 aa extracellular region, a 21 aa transmembrane region, and a 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats, many of which are also sites of calcium binding, and a cysteine-rich region just proximal to the transmembrane segment (3). Mouse Jagged 2 shares 93% and 90% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems,  gamma δ T cell lineage differentiation, and the establishment of functional natural killer cell lines (4 - 6).

1. Tsai, S. et al. (2000) Blood, 96:950.
2. Shawber, C. et al. (1996) Dev. Biol. 180:370.
3. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
4. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
5. DeHart, S. et al. (2005) Blood 105:3521.
6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.