Jagged 1 Antibody [Unconjugated]

Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP‑2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP‑2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND50 is typically 3-15 µg/mL.

• Reactivity: Rt
• Applications: WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Jagged 1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Jagged 1
  Ser32-Asp1068 (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69)
  Accession # Q63722
• Specificity: Detects rat Jagged 1 in ELISAs and Western blots. In sandwich ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2 and recombinant mouse Jagged 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: JAG1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 3-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Detection: Rat Jagged 1 Biotinylated Antibody (Catalog number BAF599) Standard: Recombinant Rat Jagged 1 Fc Chimera (Catalog number 599-JG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 1 Antibody [Unconjugated]

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

Rat Jagged 1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged 1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged 1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged 1 shows 98% and 99% aa identity to human and mouse Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%.

1. Lindsell, C.E. et al. (1995) Cell 80:909.
2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jagged 1 Antibody (AF599)(28)

Publications using AF599

• Achieng, MA;Schnell, J;Fausto, CC;Csipán, RL;Koppitch, K;Thornton, ME;Grubbs, BH;Lindström, NO; Axial nephron fate switching demonstrates a plastic system tunable on demand Nature communications 2025-08-25 [PMID: 40855070] (Immunohistochemistry, Human)
• Achieng, MA;Schnell, J;Fausto, CC;Csipán, RL;Thornton, ME;Grubbs, BH;Lindström, NO; Axial Nephron Fate Switching Demonstrates a Plastic System Tunable on Demand bioRxiv : the preprint server for biology 2025-04-02 [PMID: 40236027] (Immunohistochemistry, Human)
• Shi, M;Crouse, B;Sundaram, N;Shakked, NP;Ester, L;Zhang, W;Janakiram, V;Kopan, R;Helmrath, MA;Bonventre, JV;McCracken, KW; Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric bud bioRxiv : the preprint server for biology 2024-09-20 [PMID: 39345524] (Immunocytochemistry, Human)
• Schnell, J;Miao, Z;Achieng, M;Fausto, CC;Wang, V;Kuyper, F;Thornton, ME;Grubbs, B;Kim, J;Lindström, NO; Stepwise developmental mimicry generates proximal-biased kidney organoids bioRxiv : the preprint server for biology 2024-07-02 [PMID: 39005387] (Immunohistochemistry, Mouse)
• Liu, J;Prahl, LS;Huang, A;Hughes, AJ; Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation bioRxiv : the preprint server for biology 2024-02-08 [PMID: 38370771] (Immunohistochemistry, Mouse)
• Suda, N;Bartolomé, A;Liang, J;Son, J;Yagishita, Y;Siebel, C;Accili, D;Ding, H;Pajvani, UB; ?-cell Jagged1 is sufficient but not necessary for islet Notch activity and insulin secretory defects in obese mice Molecular metabolism 2024-02-03 [PMID: 38311286] (IHC, Mouse)
• Rios Coronado, PE;Zanetti, D;Zhou, J;Naftaly, JA;Prabala, P;Kho, PF;Martínez Jaimes, AM;Hilliard, AT;Pyarajan, S;Dochtermann, D;Million Veteran Program, ;Chang, KM;Winn, VD;Pa?ca, AM;Plomondon, ME;Waldo, SW;Tsao, PS;Clarke, SL;Red-Horse, K;Assimes, TL; CXCL12 regulates coronary artery dominance in diverse populations and links development to disease medRxiv : the preprint server for health sciences 2023-10-27 [PMID: 37961706] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Ristori, T;Thuret, R;Hooker, E;Quicke, P;Lanthier, K;Ntumba, K;Aspalter, IM;Uroz, M;Herbert, SP;Chen, CS;Larrivée, B;Bentley, K; Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe bioRxiv : the preprint server for biology 2023-09-25 [PMID: 37808725] (Human)
• Lawrence M, Elhendawi M, Morlock M et al. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity iScience 3/1/2022 [PMID: 35243244]
• E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition Cell Reports, 2021-12-14;37(11):110103. 2021-12-14 [PMID: 34910918] (IHC, Mouse, Transgenic Mouse)
• Junjie Yu, Changyu Zhu, Xiaobo Wang, KyeongJin Kim, Alberto Bartolome, Paola Dongiovanni et al. Hepatocyte TLR4 triggers inter-hepatocyte Jagged1/Notch signaling to determine NASH-induced fibrosis Science Translational Medicine 2021-06-23 [PMID: 34162749]
• Qiuyu Guo, Albert Kim, Bin Li, Andrew Ransick, Helena Bugacov, Xi Chen et al. A beta -catenin-driven switch in TCF/LEF transcription factor binding to DNA target sites promotes commitment of mammalian nephron progenitor cells eLife 2021-02-15 [PMID: 33587034] (Immunocytochemistry, Mouse)
• C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020-02-10;0(0):. 2020-02-10 [PMID: 32042114] (Western Blot, Human)
• Ransick, A, LindstrOm, N O Et al. Single-Cell Profiling Reveals Sex, Lineage, and Regional Diversity in the Mouse Kidney. Dev Cell 11/4/2019 [PMID: 31689386]
• S Wiszniak, Q Schwarz Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration BMC Neurosci, 2019-04-29;20(1):21. 2019-04-29 [PMID: 31036074] (IHC-F, Mouse)
• M Verma, Y Asakura, BSR Murakonda, T Pengo, C Latroche, B Chazaud, LK McLoon, A Asakura Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling Cell Stem Cell, 2018-10-04;23(4):530-543.e9. 2018-10-04 [PMID: 30290177] (Flow Cytometry, Mouse)
• Roxana Ola, Sandrine H. Künzel, Feng Zhang, Gael Genet, Raja Chakraborty, Laurence Pibouin-Fragner et al. SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2 Circulation 2018-11-20 [PMID: 29976569]
• Nils O. Lindström, Tracy Tran, Jinjin Guo, Elisabeth Rutledge, Riana K. Parvez, Matthew E. Thornton et al. Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning Journal of the American Society of Nephrology 2018-03-01 [PMID: 29449451]
• DW Youngstrom, R Senos, RL Zondervan, JD Brodeur, AR Lints, DR Young, TL Mitchell, ME Moore, MH Myers, WJ Tseng, KM Loomes, KD Hankenson Intraoperative delivery of the Notch ligand Jagged-1 regenerates appendicular and craniofacial bone defects NPJ Regen Med, 2017-12-15;2(0):32. 12/15/2017 [PMID: 29302365]
• Andrew H. Chang, Brian C. Raftrey, Gaetano D'Amato, Vinay N. Surya, Aruna Poduri, Heidi I. Chen et al. DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12–CXCR4 signaling axis Genes & Development 2017-07-01 [PMID: 28779009]
• PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia Nat Commun, 2016-11-29;7(0):13650. 2016-11-29 [PMID: 27897192] (IHC, Mouse)
• Wantae Kim, Sanjoy Kumar Khan, Jelena Gvozdenovic-Jeremic, Youngeun Kim, Jason Dahlman, Hanjun Kim et al. Hippo signaling interactions with Wnt/ beta -catenin and Notch signaling repress liver tumorigenesis Journal of Clinical Investigation 2016-11-21 [PMID: 27869648]
• Carrie J. Shawber, Lu Lin, Maria Gnarra, Mark V. Sauer, Virginia E. Papaioannou, Jan K. Kitajewski et al. Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus Vascular Cell 2015-12-01 [PMID: 26629328]
• Katharina S Volz, Andrew H Jacobs, Heidi I Chen, Aruna Poduri, Andrew S McKay, Daniel P Riordan et al. Pericytes are progenitors for coronary artery smooth muscle eLife 2015-10-19 [PMID: 26479710]
• Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, Shigemoto R, Shimamura K Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates. Development, 2014-04-01;141(8):1671-82. 2014-04-01 [PMID: 24715457] (IHC, Mouse)
• Anupama Gopisetty, Palash Bhattacharya, Christine Haddad, Joseph C. Bruno, Chenthamarakshan Vasu, Lucio Miele et al. OX40L/Jagged1 Cosignaling by GM-CSF–Induced Bone Marrow-Derived Dendritic Cells Is Required for the Expansion of Functional Regulatory T Cells The Journal of Immunology 2013-06-01 [PMID: 23630352]
• Bruno Larrivée, Claudia Prahst, Emma Gordon, Raquel del Toro, Thomas Mathivet, Antonio Duarte et al. ALK1 Signaling Inhibits Angiogenesis by Cooperating with the Notch Pathway Developmental Cell 2012-03-13 [PMID: 22421041]
• Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P Roles of Pofut1 and O-fucose in mammalian Notch signaling. J. Biol. Chem., 2008-03-17;283(20):13638-51. 2008-03-17 [PMID: 18347015] (Flow Cytometry, Rat)

Bioinformatics

• Gene Symbol: JAG1
• Uniprot:
  • Q63722()