Jagged 1 Antibody (188331)

Images

 
bEnd.3 mouse endo­thelioma cell line was stained with Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB1277, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by ...read more
Rat cortical stem cells were stained with Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB1277, filled histogram) or isotype control antibody (Catalog # MAB0041, open histo­gram), followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, CyTOF-ready
Clone
188331
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Jagged 1 Antibody (188331) Summary

Immunogen
NS0-derived recombinant human Jagged 1
Ser32-Asp296
Accession # P78504
Specificity
Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross‑reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry. 
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
JAG1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publications using
MAB1277 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 1 Antibody (188331)

  • AGS
  • AHDMGC104644
  • Alagille syndrome
  • AWS
  • CD339 antigen
  • CD339
  • HJ1
  • JAG1
  • Jagged 1
  • Jagged1
  • JAGL1
  • protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Jagged 1 Antibody (MAB1277)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, ICC, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(1)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB1277 Applications Species
Wafa Altalhi Diabetes impairs arterio-venous specification in engineered vascular tissues in a perivascular cell recruitment-dependent manner Biomaterials, 2016;119(0):23-32. 2016 [PMID: 27988406] (Neutralization, Human) Neutralization Human
Jagged1 Instructs Macrophage Differentiation in Leprosy PLoS Pathog, 2016;12(8):e1005808. 2016 [PMID: 27532668] (Flow Cytometry, Human) Flow Cytometry Human
Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C The CD46-Jagged1 interaction is critical for human TH1 immunity. Nat Immunol, 2012;13(12):1213-21. 2012 [PMID: 23086448] (Flow Cytometry, Human) Flow Cytometry Human
Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. J. Allergy Clin. Immunol., 2009;123(1):239-48. 2009 [PMID: 19058839] (Flow Cytometry, Human) Flow Cytometry Human
Luty WH, Rodeberg D, Parness J, Vyas YM Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates. J. Immunol., 2007;179(2):819-29. 2007 [PMID: 17617572] (ICC, Human) ICC Human
Uenotsuchi T, Takeuchi S, Matsuda T, Urabe K, Koga T, Uchi H, Fukagawa S, Kawasaki M, Kajiwara H, Moroi Y, Furue M Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence. Int. Immunol., 2006;18(12):1637-46. 2006 [PMID: 17035348] (Western Blot, Human) Western Blot Human

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1277U
Alexa Fluor 405 FAB1277V
Alexa Fluor 488 FAB1277G
Alexa Fluor 594 FAB1277T
Alexa Fluor 647 FAB1277R
Alexa Fluor 700 FAB1277N
Alexa Fluor 750 FAB1277S
Allophycocyanin FAB1277A
Biotin BAM1277
Fluorescein FAB1277F

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Bioinformatics

Gene Symbol JAG1
Uniprot