JAB1 Antibody (JB34-96)

Western Blot: JAB1 Antibody (JB34-96) [NBP2-75546] - Analysis of JAB1 on different lysate using anti-JAB1 antibody at 1/500 dilution.Positive control:Lane 1: Mouse testis tissue Lane 2: PC-3MLane 3: A549

Immunocytochemistry/Immunofluorescence: JAB1 Antibody (JB34-96) [NBP2-75546] - Staining JAB1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JAB1 Antibody (JB34-96) [NBP2-75546] - Analysis of paraffin-embedded rat brain tissue using anti-JAB1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: JAB1 Antibody (JB34-96) [NBP2-75546] - Staining JAB1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: JAB1 Antibody (JB34-96) [NBP2-75546] - Staining JAB1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JAB1 Antibody (JB34-96) [NBP2-75546] - Analysis of paraffin-embedded human kidney tissue using anti-JAB1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: JAB1 Antibody (JB34-96) [NBP2-75546] - Analysis of paraffin-embedded mouse testis tissue using anti-JAB1 antibody. Counter stained with hematoxylin.

Product Discontinued

JAB1 Antibody (JB34-96) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human JAB1 aa 1-140 / 334. (SwissProt: Q92905 Human; SwissProt: O35864 Mouse; Entrez Gene: 312916 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: COPS5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for JAB1 Antibody (JB34-96)

• COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
• COP9 signalosome complex subunit 5
• COPS5
• CSN5
• CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5
• EC 3.4
• JAB1
• JAB138 kDa Mov34 homolog
• Jun activation domain-binding protein 1
• MOV-34
• SGN5
• SGN5MGC3149
• Signalosome subunit 5

Background

The protein encoded by the JAB1 gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COPS5