ITPKB Antibody (2F8) - Azide and BSA Free

Western Blot: ITPKB Antibody (2F8) [H00003707-M01] - Analysis of ITPKB expression in transfected 293T cell line by ITPKB monoclonal antibody (M01), clone 2F8.Lane 1: ITPKB transfected lysate(67.4 KDa).Lane 2: Non-transfected lysate.

Western Blot: ITPKB Antibody (2F8) [H00003707-M01] - ITPKB monoclonal antibody (M01), clone 2F8 Analysis of ITPKB expression in Jurkat.

Sandwich ELISA: ITPKB Antibody (2F8) [H00003707-M01] - Detection limit for recombinant GST tagged ITPKB is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ITPKB Antibody (2F8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ITPKB (NP_002212, 545 a.a. ~ 643 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PELLPQDQDKPFLRKACSPSNIPAVIITDMGTQEDGALEETQGSPRGNLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPNSAFLHTLDQQKPR
• Specificity: ITPKB - inositol 1,4,5-trisphosphate 3-kinase B
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITPKB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ITPKB Antibody (2F8) - Azide and BSA Free

• EC 2.7.1.127
• inositol 14,5-trisphosphate 3-kinase BIP3K
• inositol-trisphosphate 3-kinase B
• InsP 3-kinase B
• IP3 3-kinase B
• IP3-3KB
• IP3K B
• IP3KB
• IP3K-B
• PIG37
• proliferation-inducing protein 37

Background

The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ITPKB Antibody (H00003707-M01)(1)

Publication using H00003707-M01

• Nalaskowski MM, Fliegert R, Ernst O et al. Human inositol-1,4,5-trisphosphate 3-kinase isoform B (IP3KB) is a nucleocytoplasmic shuttling protein specifically enriched at cortical actin filaments and at invaginations of the nuclear envelope. J Biol Chem. 2010-12-09 [PMID: 21148483]

Bioinformatics

• Gene Symbol: ITPKB
• Entrez:
  • 3707(Human)
• OMIM:
  • 147522(Human)
• Uniprot:
  • NP_002212(Human)