Recombinant Human ITPA Protein

SDS-Page: Recombinant Human ITPA Protein [NBP1-45292] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human ITPA Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-194 of Human ITPA
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMAASLVGKK IVFVTGNAKK LEEVVQILGD KFPCTLVAQK IDLPEYQGEP DEISIQKCQE AVRQVQGPVL VEDTCLCFNA LGGLPGPYIK WFLEKLKPEG LHQLLAGFED KSAYALCTFA LSTGDPSQPV RLFRGRTSGR IVAPRGCQDF GWDPCFQPDG YEQTYAEMPK AEKNAVSHRF RALLELQEYF GSLAA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ITPA
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Theoretical MW: 23.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human ITPA Protein

• C20orf37
• dJ794I6.3
• EC 3.6.1
• EC 3.6.1.19
• HLC14-06-P
• inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
• Inosine triphosphatase
• inosine triphosphatase-A
• inosine triphosphate pyrophosphatase
• inosine triphosphate pyrophosphohydrolase
• ITPase
• My049 protein
• nucleoside triphosphate diphosphatase
• Putative oncogene protein hlc14-06-p

Background

ITPA, also known as Inosine triphosphate pyrophosphatase, is an enzyme that catalyzes the pyrophosphohydrolysis of both ITP (inosine triphosphate) and dITP (deoxyinosine triphosphate) to IMP (inosine monophosphate) and diphosphate. IMP can be used as a substrate for purine nucleotide pathways. IMP can be phosphorylated to ITP, and ITPA can regulate the concentration of ITP in the cell by converting ITP back to IMP. Defects in ITPA result in ITPA deficiency which is thought to be inherited and is characterized by an over-accumulation of ITP in erythocytes, leukocytes and fibroblasts. Recombinant human ITPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ITPA