ITIH4 Overexpression Lysate (Native)


Western Blot: ITIH4 Overexpression Lysate (Adult Normal) [NBP2-04218] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ITIH4.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

ITIH4 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_002209. The protein contains a C-terminal DDK tag.
Homo sapiens ITIH4, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
100.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ITIH4 Overexpression Lysate (Native)

  • DKFZp686G21125
  • gp120
  • H4P
  • heavy chain-like, 1
  • IHRP
  • inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
  • inter-alpha-trypsin inhibitor family heavy chain-related protein
  • inter-alpha-trypsin inhibitor heavy chain H4
  • ITIH4
  • ITI-HC4
  • ITIHL1
  • PK120
  • PK-120
  • plasma kallikrein-sensitive glycoprotein 120
  • PRO1851


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ITIH4 Lysate (NBP2-04218). (Showing 1 - 2 of 2 FAQ).

  1. May I use this as standard in my ELISA application?
    • For our lysate NBP2-04218, we have only validated this product for use as a positive and negative control in western blot. The lysate is transfected whole cell HEK 293 cell lysate; transfected with either the target plasmid or an empty vector.
  2. What is the concentration of this lysate?
    • The whole cell lysate is provided at a concentration of 1.0mg/ml, but the exact amount of ITIH4 protein present has not been determined.

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Gene Symbol ITIH4