IRX3 Antibody (3D8) - Azide and BSA Free

Western Blot: IRX3 Antibody (3D8) [H00079191-M03] - Analysis of IRX3 expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/Immunofluorescence: IRX3 Antibody (3D8) [H00079191-M03] - Analysis of monoclonal antibody to IRX3 on HeLa cell. Antibody concentration 30 ug/ml

Western Blot: IRX3 Antibody (3D8) [H00079191-M03] - Analysis of IRX3 expression in K-562 (Cat # L009V1).

ELISA: IRX3 Antibody (3D8) [H00079191-M03] - Detection limit for recombinant GST tagged IRX3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IRX3 Antibody (3D8) - Azide and BSA Free Summary

• Immunogen: IRX3 (NP_077312, 182 a.a. ~ 285 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRRLKKENKMTWAPRSRTDEEGNAYGSEREEEDEEEDEEDCKRELELEEEELGGEEEDTGGEGLADDDEDEEIDLENLDGAATEPELSLAGAARRDGDLGLGPI
• Specificity: IRX3 - iroquois homeobox protein 3
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRX3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRX3 Antibody (3D8) - Azide and BSA Free

• Homeodomain protein IRXB1
• iroquois homeobox 3
• Iroquois homeobox protein 3
• iroquois-class homeodomain protein IRX-1
• iroquois-class homeodomain protein IRX-3
• IRX-1
• IRXB1FLJ99187

Background

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step ofneural development (Bellefroid et al., 1998 (PubMed 9427753)). Members of this family appear to play multiple rolesduring pattern formation of vertebrate embryos (Lewis et al., 1999 (PubMed 10370142)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRX3
• Entrez:
  • 79191(Human)
• Uniprot:
  • NP_077312(Human)