IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free

Western Blot: IRS1 [p Ser639, p Ser636] Antibody [NBP3-16898] - Western blot analysis of extracts of 293T cells, using IRS1 antibody (NBP3-16898) at 1:500 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic phosphorylated peptide around S636 & S639 of human IRS1 (NP_005535.1). MSPKSV
• Modification: p Ser636, p Ser639
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free

• HIRS-1
• insulin receptor substrate 1
• IRS1
• IRS-1

Background

Insulin receptor substrates (IRS), the major intracellular substrates of the insulin receptor (IR), are adaptor proteins that transduce signals from the IR to downstream effectors that are important for the biological effect of insulin (1-2). After insulin stimulation, IRS proteins are rapidly phosphorylated on multiple tyrosine residues. Once phosphorylated, IRS proteins bind and activate Grb-2, SHP2 and the PI3-K p85 subunit (2-3). IRS-1 functions as one of the key regulators of IR and the insulin-like growth factor-1 receptor. Disruption of IRS-1 causes growth retardation and insulin resistance associated with hypertension, hypertriglyceridemia, and impaired endothelium-dependent vascular relaxation (4-5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: IRS1