IRS1 Antibody - BSA Free

Western Blot: IRS1 Antibody [NBP1-77216] - PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NBP1-77216] - Immunocytochemistry of IRS1 in P815 cells with IRS1 antibody at 2 u/mL.

Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NBP1-77216] - Immunofluorescence of IRS1 in P815 cells with IRS1 antibody at 2 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IRS1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IRS1 Antibody - BSA Free (NBP1-77216) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the center of human IRS-1. The immunogen is located within amino acids 910 - 960 of IRS-1. Amino Acid Squence: QPAPREEETGTEEYMK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRS1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Western Blot 1-4 ug/ml
• Control Peptide:

  IRS1 Peptide (NBP1-77216PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IRS1 Antibody - BSA Free

• HIRS-1
• insulin receptor substrate 1
• IRS1
• IRS-1

Background

Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRS1
• Uniprot:
  • P35568()