IRS1 Antibody

Images

 
Western Blot: IRS1 Antibody [NBP1-77216] - PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: IRS1 Antibody [NBP1-77216] - P815 cells at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: IRS1 Antibody [NBP1-77216] - P815 cells with IRS-1 antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IRS1 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the center of human IRS-1. The immunogen is located within amino acids 910 - 960 of IRS-1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRS1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
IRS-1antibody can be used for the detection of IRS-1 by Western blot at 1 - 2 mg/ml.
Control Peptide
IRS1 Peptide (NBP1-77216PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRS1 Antibody

  • HIRS-1
  • insulin receptor substrate 1
  • IRS1
  • IRS-1

Background

Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IRS1 Antibody (NBP1-77216)

Discover related pathways, diseases and genes to IRS1 Antibody (NBP1-77216). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IRS1 Antibody (NBP1-77216)

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Research Areas for IRS1 Antibody (NBP1-77216)

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Blogs on IRS1.

Signalling Advances in Adiponectin Antibody Research
Adiponectin is an adipocytokine protein that positively regulates metabolism of lipids and glucose by suppressing glucose production from the liver, stimulating insulin sensitivity, and increasing the rate of fatty acid oxidation and glucose uptake. I...  Read full blog post.

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Bioinformatics

Gene Symbol IRS1
Entrez
Uniprot