IRF5 Antibody (903430) [Phycoerythrin]

A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IRF5 PE-conjugated Monoclonal Antibody (Catalog # IC8447P, filled histogram) or isotype control antibody (IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

A20 cells were stained with Rat Anti-Mouse IRF5 PE‑conjugated Monoclonal Antibody (Catalog # IC8447P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: Flow
• Clone: 903430
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

IRF5 Antibody (903430) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant mouse IRF5
  Ala208-Phe288
  Accession # P56477
• Specificity: Detects mouse IRF5 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IRF5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF5 Antibody (903430) [Phycoerythrin]

• IBD14
• interferon regulatory factor 5
• IRF5
• IRF-5
• SLEB10

Background

IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF5 Antibody (IC8447P)(2)

Publications using IC8447P

• Gschwandtner, M;Gammage, AN;Deligne, C;Mies, LFM;Domaingo, A;Murdamoothoo, D;Loustau, T;Schwenzer, A;Derler, R;Carapito, R;Koch, M;M�rgelin, M;Orend, G;Kungl, AJ;Midwood, KS; Investigating Chemokine-Matrix Networks in Breast Cancer: Tenascin-C Sets the Tone for CCL2 International journal of molecular sciences 2023-05-06 [PMID: 37176074] (Mouse)
• Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality Sci Rep, 2016-08-02;6(0):31002. 2016-08-02 [PMID: 27481535] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IRF5
• Uniprot:
  • P56477()