IRF4 Antibody (3C4)

Western Blot: IRF4 Antibody (3C4) [H00003662-M10] - Analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M10), clone 3C4. Lane 1: IRF4 transfected lysatE (51.8 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

IRF4 Antibody (3C4) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IRF4 (NP_002451, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE
• Specificity: IRF4 - interferon regulatory factor 4
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 22528235).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRF4 Antibody (3C4)

• interferon regulatory factor 4
• IRF4
• IRF-4
• LSIRF
• LSIRFMUM1
• Lymphocyte-specific interferon regulatory factor
• Multiple myeloma oncogene 1
• MUM1
• NF-EM5

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF4 Antibody (H00003662-M10)(1)

Publication using H00003662-M10

• Ni A, Chen H, Wu Y, Li W, Chen S, Li J. Expression of IRF-4 and IBP in skin lesions of patients with psoriasis vulgaris. J Huazhong Univ Sci Technolog Med Sci;32(2):287-90. 2012-04-01 [PMID: 22528235] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: IRF4
• Entrez:
  • 3662(Human)
• OMIM:
  • 601900(Human)
• Uniprot:
  • NP_002451(Human)