IRE1 alpha Antibody

Product Discontinued

IRE1 alpha Antibody Summary

• Immunogen: Peptide with sequence C-PNAHGKIKAMISD corresponding to internal region according to NP_001424.3.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ERN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human pancreas lysates (calculated MW of 110 kDa band according to NP_001424.3).
• Control:

  Human Pancreas Membrane Tissue Lysate (Adult Membrane Normal)
  Human Esophagus Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRE1 alpha Antibody

• EC 2.7.11
• endoplasmic reticulum to nucleus signaling 1
• Endoplasmic reticulum-to-nucleus signaling 1
• ER to nucleus signalling 1
• ERN1
• FLJ30999
• hIRE1p
• inositol-requiring 1
• Inositol-requiring protein 1
• IRE 1a
• IRE1 alpha
• IRE1
• IRE1a
• Ire1-alpha
• IRE1MGC163279
• IRE1P
• MGC163277
• protein kinase/endoribonuclease
• serine/threonine-protein kinase/endoribonuclease IRE1

Background

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and upregulates ER molecular chaperones in order to cope with ER stress. UPR is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha) (1). IRE1 alpha is correlated with X-box binding protein (XBP1) as a potent UPR transcriptional activator (2).

Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress (3). UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER.

IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress. IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.

References

1. Zheng, W., Xie, W., Yin, D., Luo, R., Liu, M., & Guo, F. (2019). ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling. Cell Commun Signal, 17(1), 42. doi:10.1186/s12964-019-0353-3

2. Cho, Y. M., Kim, D. H., Lee, K. H., Jeong, S. W., & Kwon, O. J. (2018). The IRE1alpha-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARgamma activity. Exp Mol Med, 50(8), 102. doi:10.1038/s12276-018-0131-0

3. Xia, Z., Wu, S., Wei, X., Liao, Y., Yi, P., Liu, Y., . . . Liu, J. (2019). Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1alpha for cell survival. J Biol Chem, 294(37), 13811-13821. doi:10.1074/jbc.RA119.008353

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRE1 alpha Antibody (NBP1-05040)(1)

Publication using NBP1-05040

• Yokouchi M, Hiramatsu N, Hayakawa K et al. Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response. J Biol Chem 2007-12-17 [PMID: 18086661]

Review for IRE1 alpha Antibody (NBP1-05040) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human, mouse(test) and human (control) whole cell lysate, Mouse.

reviewed by: Qian Wang WB Human, mouse(test) and human (control) whole cell lysate, Mouse 04/10/2017

Summary

• Application: Western Blot
• Sample Tested: HEK 293 cell line,hela cell,homemade mouse embryonic fibroblast,NIH 3T3 cells,whole cell lysate
• Species: Human, mouse(test) and human (control) whole cell lysate, Mouse
• Lot: G2E011014

Comments

• Comments: Procedures:
  Cell sample were collected and lysated with RIPA
  total protein concentration determined with Bio-Rad protein assay
  Whole cell lysates were load to SDS-PAGE with 6% stacking and 10% resolving
  Gel then transferred to nitrocellulose membrane at 4 degrees, overnight.
  WB: membrane first blotted with Novusbio IRE1 antibody (1:1000 dilution), secondary antibody used rabbit anti-goat (1:2000 dilution).
  Membrane then washed (no stripping) and blotted with GAPDH (abcam, ab9485, 1:1000), with secondary antibody mouse anti rabbit (1:2000 dilution)

Bioinformatics

• Gene Symbol: ERN1
• Entrez:
  • 2081(Human)
  • 78943(Mouse)
  • 498013(Rat)
• Uniprot:
  • NP_001424.3(Human)