IRE1 alpha Antibody


Western Blot: IRE1 alpha Antibody [NB110-59971] - Analysis using the Biotin conjugate of NB110-59971. Detection of LPS+Tm treated macrophages. Image from verified customer review.
Western Blot: IRE1 alpha Antibody [NB110-59971] - Detection of IRE-1 alpha using NB110-59971. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB
0.9 mg/ml

IRE1 alpha Antibody Summary

A synthetic peptide within the human IRE1 alpha protein (within residues 200-300). [Swiss-Prot #O75460]
Endoplasmic reticulum
Immunogen affinity purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
0.9 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000
Application Notes
This IRE1 alpha antibody is useful for Western blot, where a band can be seen at ~110 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa
Control Peptide
IRE1 alpha Peptide (NB110-59971PEP)

Reactivity Notes

Human and mouse.

Alternate Names for IRE1 alpha Antibody

  • EC 2.7.11
  • endoplasmic reticulum to nucleus signaling 1
  • Endoplasmic reticulum-to-nucleus signaling 1
  • ER to nucleus signalling 1
  • FLJ30999
  • hIRE1p
  • inositol-requiring 1
  • Inositol-requiring protein 1
  • IRE 1a
  • IRE1a
  • ire1-alpha
  • IRE1MGC163279
  • IRE1P
  • MGC163277
  • protein kinase/endoribonuclease
  • serine/threonine-protein kinase/endoribonuclease IRE1


Unfolded protein response (UPR) signaling, mechanism used by eukaryotic cells to cope ER stress, is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha). UPR-responsive genes's transcriptional activation is regulated by ATF6 and IRE1-XBP1 pathways, and UPR serves three important functions: inhibition of protein translation to restore normal cell functions; activation of signaling to increase production of molecular chaperones involved in protein folding; and activation of signaling that leads to targeting of misfolded proteins in ER for ubiquitination and subsequent degradation; or when ER-stress is not relieved, UPR leads to apoptosis. Localized as a single-pass type I membrane protein in ER membrane, IRE1 alpha is ubiquitously expressed with high levels observed in pancreatic tissue. It undergoes autophosphorylation and gets ADP-ribosylated by PARP16 upon ER stress, which increases its kinase as well as endonuclease activities. IRE1 alpha senses unfolded proteins in the ER lumen via its N-terminal domain which leads to enzyme auto-activation and thereafter, the active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent UPR transcriptional activator. IRE1 alpha inactivation in mice has been shown to result in widespread developmental defects, leading to intra-gestational embryonic lethality and ER stress mediated cell death is responsible for several pathologies.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-59971AF405
Alexa Fluor (R) 488 Labeled NB110-59971AF488
Alexa Fluor (R) 647 Labeled NB110-59971AF647
Alexa Fluor (R) 700 Labeled NB110-59971AF700
Biotin Labeled NB110-59971B
DyLight 405 Labeled NB110-59971V
DyLight 488 Labeled NB110-59971G
DyLight 550 Labeled NB110-59971R
DyLight 650 Labeled NB110-59971C
FITC Labeled NB110-59971F
HRP Labeled NB110-59971H

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Blogs on IRE1 alpha.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

IRE1 alpha (inositol-requiring enzyme 1 alpha)
The unfolded protein response (UPR) is a eukaryotic cell process that addresses ER stress. UPR is initiated by three ER-localized sensors: PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 alpha (...  Read full blog post.

IRE1 alpha and ER Stress: Keys to Disease Progression Pathways
Inositol Requiring Enzyme 1 alpha (IRE1 alpha) is a transmembrane-RNase with an endoplasmic reticulum (ER) luminal sensor domain and cytosolic kinase and ribonuclease domains. IRE1 also plays a central role in the ER stress response (1). In a recent s...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol ERN1

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