IQGAP1 Antibody (JG38-74)

Images

 
Western Blot: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HUVEC Lane 2: A431 Lane 3: Mouse placenta
Immunocytochemistry/ Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded mouse colon tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.
Flow Cytometry: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Flow cytomeAnalysis of SiHa cells with IQGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary ...read more
Immunocytochemistry/ Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded human lung cancer tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded human spleen tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded human placenta tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JG38-74
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IQGAP1 Antibody (JG38-74) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein corresponding to N-terminal human IQGAP1.
Localization
Cell membrane. Cytoplasm. Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IQGAP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
189 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IQGAP1 Antibody (JG38-74)

  • HUMORFA01
  • IQ motif containing GTPase activating protein 1
  • IQGAP1
  • KIAA0051ras GTPase-activating-like protein IQGAP1
  • p195
  • p195RasGAP-like with IQ motifs
  • SAR1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IQGAP1