IQGAP1 Antibody (JG38-74)

Western Blot: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HUVEC Lane 2: A431 Lane 3: Mouse placenta

Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded mouse colon tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.

Flow Cytometry: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Flow cytomeAnalysis of SiHa cells with IQGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Staining IQGAP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded human lung cancer tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded human spleen tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IQGAP1 Antibody (JG38-74) [NBP2-75545] - Analysis of paraffin-embedded human placenta tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.

Product Discontinued

IQGAP1 Antibody (JG38-74) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human IQGAP1 aa 10-100 / 1657. (SwissProt: P46940 Human; SwissProt: Q9JKF1 Mouse)
• Localization: Cell membrane. Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IQGAP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 189 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IQGAP1 Antibody (JG38-74)

• HUMORFA01
• IQ motif containing GTPase activating protein 1
• IQGAP1
• KIAA0051ras GTPase-activating-like protein IQGAP1
• p195
• p195RasGAP-like with IQ motifs
• SAR1

Background

IQGAP1 encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IQGAP1