Involucrin Antibody (SY5)

Product Discontinued

Involucrin Antibody (SY5) Summary

• Immunogen: Purified involucrin from human keratinocytes
• Localization: Cytoplasmic
• Marker: Squamous Cell Terminal Differentiation Marker
• Specificity: It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IVL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
  • Western Blot 0.5-1.0ug/ml
• Application Notes: Human Chromosome Location: 1q21.3
  Mol. Weight of Antigen: 66-170kDa

Reactivity Notes

Gorilla, Owl Monkey

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Involucrin Antibody (SY5)

• involucrin

Background

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Involucrin Antibody (NBP2-34264) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 12/08/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human skin
• Species: Human
• Lot: 37131p160623

Bioinformatics

• Gene Symbol: IVL
• Uniprot:
  • P07476(Human)