INTS8 Antibody - BSA Free

Western Blot: INTS8 Antibody [NB100-459] - Detection of Human FLJ20530/KAONASHI1 by Western Blot. Samples : Nuclear extract (NE) from HeLa cells. Antibody : Affinity purified rabbit anti-FLJ20530/KAONASHI1 antibody used at 0.2 ug/ml. Detection : Chemiluminescence with an exposure time of 20 minutes.

Immunohistochemistry: INTS8 Antibody [NB100-459] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- FLJ20530/KAONASHI1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: INTS8 Antibody [NB100-459] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-INT4 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

INTS8 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 200 and 250 of human FLJ20530/KAONASHI1 using the numbering given in TrEMBL entry Q7Z314 (GeneID 55656).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INTS8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Western Blot 1:2500-1:15000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for INTS8 Antibody - BSA Free

• C8orf52
• FLJ20530
• INT8
• integrator complex subunit 8
• protein kaonashi-1

Background

FLJ20530 is a component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3' box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INTS8
• Entrez:
  • 55656(Human)
• Uniprot:
  • Q7Z314(Human)