Recombinant Human Interferon beta 1b Protein

Product Discontinued

Recombinant Human Interferon beta 1b Protein Summary

• Description: A single, non-glycosylated biologically active polypeptide chain corresponding to 166 residues of IFNB1.
  
  

  Source: E. coli

  Amino Acid Sequence: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIYEMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSLHLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN

• Details of Functionality: Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 3.0 x 107 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IFNB1
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Endotoxin as determined by LAL method

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 20.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, containing 2 % HSA and 3 % mannitol.
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Background

IFN-betas are proteins produced by many cell types including lymphocytes (NK cells, B-cells and T-cells), macrophages, fibroblasts, endothelial cells, osteoblasts and others. They have antiviral activity that it is mainly involved in innate immune response. The IFN-beta family has 2 subtypes,which are IFN-beta1 (IFNB1) and IFN-beta3 (IFNB3) (a gene designated IFN-beta2 is actually IL-6). IFN-beta1 is used as a treatment for multiple sclerosis as it reduces the relapse rate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNB1
• Uniprot:
  • P01574()
  • P01574()
  • P01574()