Intelectin-2 Antibody

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Intelectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5358) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Intelectin-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Intelectin-2 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Intelectin-2
  Ala20-Ser298
  Accession # Q80ZA0
• Specificity: Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Intelectin-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Itlnb
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-2 Antibody

• HL-2
• intelectin 2
• Intelectin2
• Intelectin-2
• Intelectin-b
• Itln1b
• ITLN2
• Itlnb
• UNQ2789/PRO7179

Background

Intelectin-2 (ITLN-2, ITLN-1b and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by airway mucous cells plus intestinal Paneth and goblet cells, and appears to participate in fungal microbe recognition. The mouse ITLN-2 precursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature region (aa 20-298), and a GPI-linked prodomain (aa 299-313). The mature region contains one C-terminal type fibrinogen domain (aa 32-251). Over aa 20-298, mouse ITLN-2 shares 79% aa sequence identity with human ITLN-2, and 92% aa sequence identity with mouse ITLN-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Itlnb
• Uniprot:
  • Q80ZA0()