Intelectin-2 Antibody (747421)

Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intelectin-2 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 747421
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Intelectin-2 Antibody (747421) Summary

• Immunogen: E. coli-derived recombinant mouse Intelectin-2
  Ala20-Ser298
  Accession # Q80ZA0
• Specificity: Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) Intelectin-1 is observed. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) Intelectin-2 is observed, and approximately 15% cross-reactivity with rhIntelectin-1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Itlnb
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-2 Antibody (747421)

• HL-2
• intelectin 2
• Intelectin2
• Intelectin-2
• Intelectin-b
• Itln1b
• ITLN2
• Itlnb
• UNQ2789/PRO7179

Background

Intelectin-2 (endothelial lectin 2; also ITLN-2 and HL-2) is a 40 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells and appears to participate in fungal microbe recognition. The Intelectin-2 precursor is 325 amino acids (aa) in length. It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse Intelectin-2 is reportedly GPI-linked and mouse and human Intelectin-2 show considerable C‑terminal aa identity, it is not known if human Intelectin-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 45‑210). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 20‑298, mouse Intelectin-2 shows 78% aa identity to human Intelectin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Itlnb
• Uniprot:
  • Q80ZA0()